Wang Lei, Chen Chen, Li Fen, Hua Qingquan, Chen Shiming, Xiao Bokui, Dai Mengyuan, Li Man, Zheng Anyuan, Yu Di, Hu Zhangwei, Tao Zezhang
Department of Otolaryngology Head and Neck Surgery, Renmin Hospital of Wuhan University Wuhan 430060, PR China.
Department of Research Institute of Otolaryngology Head and Neck Surgery, Renmin Hospital of Wuhan University Wuhan 430060, PR China.
Int J Clin Exp Pathol. 2015 Aug 1;8(8):8857-68. eCollection 2015.
To examine the significance of the Neutrophil gelatinase-associated lipocalin (NGAL) in diagnosing head and neck squamous cell carcinoma (HNSCC) and predicting regional metastasis. We first used GEO dataset to analyze the NGAL gene expression in HNSCC. Then, we summarized the characteristics of patients retrospectively selected in clinic. Expression of NGAL protein in human HNSCC tumor, lymph node and normal samples were analyzed using immunohistochemistry. Next, we further investigated the NGAL expression in a tissue microassay to analyze the relationship between NGAL protein expression and TNM stage. Finally, we tested the NGAL protein expression in head and neck cancer cell lines. Analysis of GEO dataset concluded that NGAL gene expression in HNSCC was lower than that in normal tissue (P<0.01). There was no statistically significant difference of NGAL gene expression between T-stage and N-stage (P>0.05). NGAL protein expression in tumor was lower than that in normal tissue (P<0.01). There was no statistically significant difference of NGAL protein expression between metastasis group and non-metastasis group (P>0.05). Expression of NGAL protein was not correlated with TNM stage of HNSCC. Aggressive HNSCC cell lines have lower NGAL protein expression. Our data demonstrated that the expression of NGAL protein was correlated with tumorigenesis of HNSCC, but not with regional metastasis. It may serve as a novel biomarker for prognostic evaluation of patients with HNSCC.
为研究中性粒细胞明胶酶相关脂质运载蛋白（NGAL）在诊断头颈部鳞状细胞癌（HNSCC）及预测区域转移中的意义。我们首先使用基因表达综合数据库（GEO）数据集分析HNSCC中NGAL基因的表达。然后，我们回顾性总结了临床中选取患者的特征。采用免疫组织化学方法分析人HNSCC肿瘤、淋巴结及正常样本中NGAL蛋白的表达。接下来，我们在组织芯片中进一步研究NGAL的表达，以分析NGAL蛋白表达与TNM分期之间的关系。最后，我们检测了头颈部癌细胞系中NGAL蛋白的表达。对GEO数据集的分析得出，HNSCC中NGAL基因表达低于正常组织（P<0.01）。T分期和N分期之间NGAL基因表达无统计学显著差异（P>0.05）。肿瘤中NGAL蛋白表达低于正常组织（P<0.01）。转移组和非转移组之间NGAL蛋白表达无统计学显著差异（P>0.05）。NGAL蛋白表达与HNSCC的TNM分期无关。侵袭性HNSCC细胞系的NGAL蛋白表达较低。我们的数据表明，NGAL蛋白表达与HNSCC的肿瘤发生相关，但与区域转移无关。它可能作为HNSCC患者预后评估的一种新型生物标志物。
