Fang Ying, Ren Xiaoxia, Feng Zhanwei
Department of Gastroenterology, Xi'an Children's Hospital Shaanxi 710002, China.
Department of Respiratory, Xi'an Children's Hospital Shaanxi 710002, China.
Int J Clin Exp Pathol. 2015 Aug 1;8(8):9586-91. eCollection 2015.
In order to explore the relevance of SOCS3 gene polymorphisms with infantile asthma and provide evidence for the ethology of infantile asthma, we conducted this case-control study.
A total of 273 children were enrolled for study in this article, including 119 children with asthma and 154 healthy controls frequency-matched with the former in sex and age. The genotyping of SOCS3 rs4969170, rs4969168 polymorphisms in all subjects were performed using TaqMan probe method. Odds ratio (OR) with 95% confidence interval (CI) was used to represent the association strength between SOCS3 polymorphisms and infantile asthma and calculated by χ² test which was conducted to check the Hardy-Weinberg equilibrium (HWE) in the control group.
The genotypes distributions of SOCS3 polymorphisms in controls conformed to HWE. Compared with GG/GA genotype in SOCS3 rs4969170, AA genotype obviously increased the susceptibility to asthma in children (OR=2.556, 95% CI=1.377-4.744) and A allele also made the same conclusion (OR=2.287, 95% CI=1.311-3.991). Differently in rs4969168, AG and AG/GG genotypes distributions had significant differences in two groups (P=0.036, 0.043). This two polymorphisms existed the linkage disequilibrium and the haplotype analysis showed that A-G and A-A haplotypes in rs4969170-rs4969168 increased 1.855 and 0.863 times risk of asthma development in children, respectively.
A significant relevance involved in SOCS3 gene polymorphisms and infantile asthma development based on a Chinese Han population.
为探讨细胞因子信号转导抑制因子3（SOCS3）基因多态性与婴幼儿哮喘的相关性，为婴幼儿哮喘的病因学提供依据，我们开展了这项病例对照研究。
本文共纳入273名儿童进行研究，其中119名哮喘患儿和154名年龄及性别频率匹配的健康对照。采用TaqMan探针法对所有受试者的SOCS3 rs4969170、rs4969168多态性进行基因分型。采用比值比（OR）及95%置信区间（CI）表示SOCS3多态性与婴幼儿哮喘的关联强度，并通过χ²检验计算，该检验用于检验对照组的哈迪-温伯格平衡（HWE）。
对照组中SOCS3多态性的基因型分布符合HWE。与SOCS3 rs4969170的GG/GA基因型相比，AA基因型显著增加了儿童患哮喘的易感性（OR = 2.556，95%CI = 1.377 - 4.744），A等位基因也得出相同结论（OR = 2.287，95%CI = 1.311 - 3.991）。不同的是，在rs4969168中，AG和AG/GG基因型分布在两组间有显著差异（P = 0.036，0.043）。这两个多态性存在连锁不平衡，单倍型分析显示，rs4969170 - rs4969168中的A - G和A - A单倍型分别使儿童哮喘发病风险增加1.855倍和0.863倍。
基于中国汉族人群，SOCS3基因多态性与婴幼儿哮喘发病显著相关。
