Nakazawa M M, Silva Júnior W R S, Kato M T, Gavazza S, Florencio L
Laboratório de Saneamento Ambiental, Departamento de Engenharia Civil, Universidade Federal de Pernambuco. Av. Acadêmico Hélio Ramos, s/n. Cidade Universitária. CEP: 50740-530 Recife - PE, Brazil E-mail:
Laboratório de Saneamento, Campus Afogados da Ingazeira, Instituto Federal de Educação, Ciência e Tecnologia de Pernambuco. Sítio Campinhos, s/n. CEP: 56800-000 Afogados da Ingazeira - PE, Brazil.
Water Sci Technol. 2015;72(8):1383-9. doi: 10.2166/wst.2015.345.
In this study, we evaluated the use of an up-flow anaerobic sludge blanket (UASB) reactor to treat crude glycerol obtained from cottonseed biodiesel production. The laboratory-scale UASB reactor (7.0 L) was operated at ambient temperature of 26.5°C with chemical oxygen demand (COD) concentrations between 0.5 and 8.0 g/L. The volatile fatty acid contents, pH, inorganic salt contents and biogas production were monitored during a 280-day experimental period. Molecular biology techniques were used to assess the microbial diversity in the bioreactor. The reactor achieved COD removal efficiencies of up to 92% except during one phase when the efficiency decreased to 81%. Biogas production remained stable throughout the experimental period, when the fraction converted to methane reached values as high as 68%. The profile of the denaturing gradient gel electrophoresis (DGGE) bands suggested slight changes in the microbial community during reactor operation. The overall results indicated that the crude glycerol from biodiesel production can serve as a suitable substrate for anaerobic degradation with a stable reactor performance and biogas production as long as the applied organic loads are up to 8.06 kg COD/m3·d.
在本研究中，我们评估了上流式厌氧污泥床（UASB）反应器用于处理从棉籽生物柴油生产中获得的粗甘油的情况。实验室规模的UASB反应器（7.0升）在26.5°C的环境温度下运行，化学需氧量（COD）浓度在0.5至8.0克/升之间。在280天的实验期内监测挥发性脂肪酸含量、pH值、无机盐含量和沼气产量。使用分子生物学技术评估生物反应器中的微生物多样性。该反应器除了在一个阶段效率降至81%外，COD去除效率高达92%。在整个实验期内沼气产量保持稳定，转化为甲烷的比例高达68%。变性梯度凝胶电泳（DGGE）条带图谱表明，在反应器运行期间微生物群落略有变化。总体结果表明，只要施加的有机负荷高达8.06千克COD/立方米·天，生物柴油生产中的粗甘油可作为厌氧降解的合适底物，反应器性能和沼气产量稳定。
