Laboratory of Sustainable Environmental Biology, Graduate School of Agricultural Science, Tohoku University, Yomogida 232-3, Naruko-onsen, Osaki, Miyagi 989-6711, Japan; Dept. of Civil and Environmental Engineering, Tohoku University, 6-6-06 Aoba, Sendai, Miyagi 980-8579, Japan.
Bioresour Technol. 2013 Dec;150:387-92. doi: 10.1016/j.biortech.2013.10.030. Epub 2013 Oct 16.
The use of Japanese cedar charcoal as a support material for microbial attachment could enhance methane production during anaerobic digestion of crude glycerol and wastewater sludge. Methane yield from a charcoal-containing reactor was approximately 1.6 times higher than that from a reactor without charcoal, and methane production was stable over 50 days when the loading rate was 2.17 g chemical oxygen demand (COD) L(-1) d(-1). Examination of microbial communities on the charcoal revealed the presence of Uncultured Desulfovibrio sp. clone V29 and Pelobacter seleniigenes, known as 1,3-propandiol degraders. Hydrogenotrophic methanogens were also detected in the archaeal community on the charcoal. Methanosaeta, Methanoregula, and Methanocellus were present in the charcoal-containing reactor. The concentration of propionate in the charcoal-containing reactor was also lower than that in the control reactor. These results suggest that propionate degradation was enhanced by the consumption of hydrogen by hydrogenotrophic methanogens on the charcoal.
利用日本扁柏木炭作为微生物附着的支撑材料，可以提高粗甘油和废水污泥厌氧消化过程中的甲烷产量。含木炭的反应器的甲烷产量比不含木炭的反应器高约 1.6 倍，当负荷率为 2.17 g 化学需氧量（COD）L(-1)d(-1)时，甲烷产量稳定在 50 天以上。对木炭上微生物群落的检查表明，存在未培养脱硫弧菌克隆 V29 和 Pelobacter seleniigenes，它们是 1,3-丙二醇降解菌。在木炭上的古菌群落中也检测到了氢营养型产甲烷菌。在含木炭的反应器中存在甲烷八叠球菌、甲烷调节菌和甲烷微球菌。含木炭的反应器中丙酸的浓度也低于对照反应器。这些结果表明，木炭上的氢营养型产甲烷菌消耗氢气，促进了丙酸的降解。
