Pérez-Legaspi I A, Rico-Martínez R, Quintanar J L
División de Estudios de Posgrado e Investigación, Instituto Tecnológico de Boca del Río, MX.
Departamento de Química, Centro de Ciencias Básicas, Universidad Autónoma de Aguascalientes, MX.
Braz J Biol. 2015 Aug;75(3):759-65. doi: 10.1590/1519-6984.01614. Epub 2015 Aug 25.
The organophosphate and carbamate pesticides methyl-parathion and carbaryl have a common action mechanism: they inhibit acetylcholinesterase enzyme by blocking the transmission of nerve impulses. However, they can alter the expression of exocytotic membrane proteins (SNARE), by modifying release of neurotransmitters and other substances. This study evaluated the adverse effects of the pesticides methyl-parathion and carbaryl on expression of SNARE proteins: Syntaxin-1, Syntaxin-4 and SNAP-23 in freshwater rotifer Brachionus calyciflorus. Protein expression of these three proteins was analyzed before and after exposure to these two pesticides by Western Blot. The expression of Syntaxin-1, Syntaxin-4 and SNAP-23 proteins in B. calyciflorussignificantly decreases with increasing concentration of either pesticides. This suggests that organophosphates and carbamates have adverse effects on expression of membrane proteins of exocytosis by altering the recognition, docking and fusion of presynaptic and vesicular membranes involved in exocytosis of neurotransmitters. Our results demonstrate that the neurotoxic effect of anticholinesterase pesticides influences the interaction of syntaxins and SNAP-25 and the proper assembly of the SNARE complex.
它们通过阻断神经冲动的传递来抑制乙酰胆碱酯酶。然而，它们可以通过改变神经递质和其他物质的释放来改变胞吐膜蛋白（SNARE）的表达。本研究评估了农药甲基对硫磷和西维因对淡水轮虫萼花臂尾轮虫中SNARE蛋白（Syntaxin-1、Syntaxin-4和SNAP-23）表达的不利影响。通过蛋白质免疫印迹法分析了这两种农药暴露前后这三种蛋白的表达情况。随着任一种农药浓度的增加，萼花臂尾轮虫中Syntaxin-1、Syntaxin-4和SNAP-23蛋白的表达显著降低。这表明有机磷酸酯类和氨基甲酸酯类农药通过改变神经递质胞吐过程中涉及的突触前膜和囊泡膜的识别、对接和融合，对胞吐膜蛋白的表达产生不利影响。我们的结果表明，抗胆碱酯酶农药的神经毒性作用会影响Syntaxin和SNAP-25的相互作用以及SNARE复合体的正确组装。
