Universidad Nacional Autónoma de México, Posgrado en Ciencias del Mar y Limnología, Ciudad Universitaria, Mexico.
Biomarkers. 2011 Feb;16(1):12-9. doi: 10.3109/1354750X.2010.520336. Epub 2010 Oct 27.
The inhibitory effects on esterases and phospholipase A2 (PLA2) in the freshwater rotifer Euchlanis dilatata, native to Mexico, were assessed by fluorimetry after in vivo exposure (30 min) in laboratory conditions to sublethal concentrations of metals and pesticides. EC(50) values for esterases ranged from 7.9 × 10(-7) for DDT to 61.9 μg l(-1) for methyl parathion, while corresponding values for PLA2 ranged from 0.96 × 10(-6) for mercury to 69.2 μg l(-1) for lead. These enzyme systems in E. dilatata are very sensitive to the tested agents and suggest they would be suitable biomarkers. However, sensitivity to other environmental contaminants should be investigated in laboratory conditions and field studies to assess their potential as environmental biomarkers.
采用氟化学发光法检测了原产于墨西哥的淡水轮虫 Euchlanis dilatata 在实验室条件下经亚致死浓度的金属和农药体内暴露 30 分钟后对酯酶和磷脂酶 A2（PLA2）的抑制作用。酯酶的 EC50 值范围从滴滴涕（DDT）的 7.9×10（-7）到甲基对硫磷的 61.9μg/L，而 PLA2 的相应值范围从汞的 0.96×10（-6）到铅的 69.2μg/L。E. dilatata 中的这些酶系统对测试剂非常敏感，表明它们将是合适的生物标志物。然而，应该在实验室条件和野外研究中调查对其他环境污染物的敏感性，以评估它们作为环境生物标志物的潜力。
