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刺柏(柏科)叶绿体全基因组的特征分析

Characterization of the complete chloroplast genome of Juniperus cedrus (Cupressaceae).

作者信息

Guo Qiqiang, Bianba Duoji, Zheng Weilie

机构信息

a Agricultural and Animal Husbandry College, Tibet University , Nyingchi , Tibet , China.

b Key Laboratory of Forest Ecology in Tibet Plateau (Tibet University) Ministry of Education , Nyingchi , Tibet , China.

出版信息

Mitochondrial DNA A DNA Mapp Seq Anal. 2016 Nov;27(6):4355-4356. doi: 10.3109/19401736.2015.1089498. Epub 2015 Oct 14.

Abstract

The complete chloroplast genome of Juniperus cedrus is a circular molecule of 127 126 bp in length with 115 single copy genes and two duplicated genes (trnI-CAU and trnQ-UUG). The genome contains 82 protein-coding genes, four ribosomal RNA genes and 33 transfer RNA genes. In these genes, eight genes (rpl16, rpl2, rpoC1, petD, petB, ndhB, ndhA and atpF) harbor a single intron and two genes (rps12 and ycf3) harbor two introns. Like other sequenced chloroplast genomes of conifers, this genome does not contain canonical inverted repeats (IRs), and the overall GC content of J. cedrus chloroplast DNA is 35%. The phylogenetic analysis revealed that J. cedrus is more closely related to J. scopulorum and J. bermudiana.

摘要

刺柏的完整叶绿体基因组是一个长度为127126 bp的环状分子,包含115个单拷贝基因和两个重复基因(trnI-CAU和trnQ-UUG)。该基因组包含82个蛋白质编码基因、4个核糖体RNA基因和33个转运RNA基因。在这些基因中,8个基因(rpl16、rpl2、rpoC1、petD、petB、ndhB、ndhA和atpF)含有一个内含子,2个基因(rps12和ycf3)含有两个内含子。与其他已测序的针叶树叶绿体基因组一样,该基因组不包含典型的反向重复序列(IRs),刺柏叶绿体DNA的总体GC含量为35%。系统发育分析表明,刺柏与落基山刺柏和百慕大刺柏的亲缘关系更近。

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