Nah Gyoungju, Im Ji-Hoon, Lim Soo-Hyun, Kim Kyunghee, Choi Ah Young, Yook Min Jung, Kim Soonok, Kim Changmu, Kim Do-Soon
a Department of Plant Science , College of Agriculture and Life Sciences, Research Institute of Agriculture and Life Sciences, Seoul National University , Seoul , Korea.
b College of Agriculture and Life Sciences, National Instrumentation Center for Environment Management (NICEM), Seoul National University , Seoul , Korea , and.
Mitochondrial DNA A DNA Mapp Seq Anal. 2016 Nov;27(6):4359-4360. doi: 10.3109/19401736.2015.1089500. Epub 2015 Oct 14.
The complete chloroplast (cp) genomes of two Miscanthus species, M. sinensis and M. sacchariflorus, were sequenced and investigated for genes, genome size variation, and polymorphisms. There are 170 genes in both cp genomes, consisting of 122 mRNA genes (84 protein-coding genes and 38 hypothetical genes), 40 tRNA genes, and 8 rRNA genes. The cp genome contains two inverted repeat (IR) regions, separated by large single copy (LSC) region and small single copy (SSC) region. Indels were responsible for 40 bp difference in cp genome size in two species. In addition, we established phylogenetic relationship with other monocot cp genomes, and estimated divergence time. The two Miscanthus species clustered together among other C monocot species and the divergence time of two Miscanthus species was approximately 0.5 1-0.84 Mya.
对芒草属的两个物种——中国芒（M. sinensis）和荻（M. sacchariflorus）的完整叶绿体（cp）基因组进行了测序，并对其基因、基因组大小变异和多态性进行了研究。两个cp基因组中均有170个基因，包括122个mRNA基因（84个蛋白质编码基因和38个假定基因）、40个tRNA基因和8个rRNA基因。cp基因组包含两个反向重复（IR）区域，由大单拷贝（LSC）区域和小单拷贝（SSC）区域隔开。插入缺失导致两个物种的cp基因组大小相差40 bp。此外，我们与其他单子叶植物cp基因组建立了系统发育关系，并估计了分化时间。这两个芒草属物种在其他C类单子叶植物物种中聚在一起，两个芒草属物种的分化时间约为0.51-0.84百万年前。
