Liu Xiao-Ying, Udayanga Dhanushka, Luo Zong-Long, Chen Li-Jiao, Zhou De-Qun, Su Hong Yan, Hyde Kevin D
College of Basic Medicine, Dali University, Dali, Yunnan 671000, China; College of Agriculture and Biology, Dali University, Dali, Yunnan 671003, China.
Center of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai, 57100, Thailand; School of Science, Mae Fah Luang University, Chiang Rai, 57100, Thailand.
Fungal Biol. 2015 Nov;119(11):1046-1062. doi: 10.1016/j.funbio.2015.08.005. Epub 2015 Aug 12.
We are studying the freshwater lignicolous fungi along a north-south latitudinal gradient in Asia. In this paper, fresh collections of Minimelanolocus from submerged wood in streams in Yunnan Province, China are characterised based on morphology and molecular phylogeny based on three rDNA regions: 18S (SSU), ITS1-5.8S-ITS2 (ITS) and 28S nuclear rDNA (LSU). The phylogenetic analysis of combined LSU and SSU sequence data and a separate analysis of ITS placed the isolates within the family Herpotrichiellaceae, order Chaetothyriales. An updated phylogenetic backbone tree for Chaetothyriales is provided with available ex-type and additional isolates. One of the isolates collected was identified as Minimelanolocus obscurus based on morphology and molecular data. Minimelanolocus aquaticus, M. asiaticus, M. curvatus and M. melanicus are described as new species considering the interspecific ITS variability and morphology. The phylogenetic placement of Minimelanolocus in Chaetothyriales is novel and provides new sequence data for the genus as a distinct lineage in Chaetothyriales. The conidial characters of all the known species in the genus are summarized. Descriptions and illustrations are provided for the five species of Minimelanolocus with notes on their taxonomy and phylogeny.
我们正在研究亚洲沿南北纬度梯度分布的淡水木生真菌。在本文中，基于形态学以及基于三个rDNA区域（18S（小亚基）、ITS1 - 5.8S - ITS2（ITS）和28S核rDNA（大亚基））的分子系统发育，对采自中国云南省溪流中淹没木材上的微小黑团孢属新鲜样本进行了特征描述。对联合的大亚基和小亚基序列数据进行的系统发育分析以及对ITS的单独分析将分离株置于毛壳菌科、毛壳目内。提供了一个更新的毛壳目系统发育骨干树，其中包含可用的模式菌株和其他分离株。根据形态学和分子数据，所采集的一个分离株被鉴定为暗微小黑团孢。考虑到种间ITS变异性和形态学，水生微小黑团孢、亚洲微小黑团孢、弯微小黑团孢和黑微小黑团孢被描述为新物种。微小黑团孢在毛壳目中的系统发育位置是新颖的，并为该属作为毛壳目中一个独特的谱系提供了新的序列数据。总结了该属所有已知物种的分生孢子特征。提供了微小黑团孢五个物种的描述和图示，并对其分类学和系统发育进行了说明。
