Guangxi University of Chinese Medicine, Nanning, 530200, China.
Guangxi Botanical Garden of Medicinal Plant, Nanning, 530023, China.
Sci Rep. 2018 Apr 12;8(1):5929. doi: 10.1038/s41598-018-24313-2.
The plant Dysosma versipellis is known for its antimicrobial and anticancer properties but is a rare and vulnerable perennial herb that is endemic to China. In this study, 224 isolates were isolated from various tissues of D. versipellis, and were classified into 53 different morphotypes according to culture characteristics and were identified by sequence analyses of the internal transcribed spacer (ITS) region of the rRNA gene. Although nine strains were not assignable at the phylum level, 44 belonged to at least 29 genera of 15 orders of Ascomycota (93%), Basidiomycota (6%), and Zygomycota (1%). Subsequent assays revealed antimicrobial activities of 19% of endophytic extracts against at least one pathogenic bacterium or fungus. Antimicrobial activity was also determined using the agar diffusion method and was most prominent in extracts from four isolates. Moreover, high performance liquid chromatography (HPLC) and ultra-performance liquid chromatography-quadrupole-time of flight mass spectrometry analyses (UPLC-QTOF MS) showed the presence of podophyllotoxin in two Fusarium strains, with the highest yield of 277 μg/g in Fusarium sp. (WB5121). Taken together, the present data suggest that various endophytic fungi of D. versipellis could be exploited as sources of novel natural antimicrobial or anticancer agents.
药用植物八角莲（Dysosma versipellis）具有抗菌和抗癌特性，但它是一种罕见且脆弱的中国特有的多年生草本植物。在这项研究中，从八角莲的各种组织中分离出 224 株分离株，根据培养特征将其分为 53 种不同的形态型，并通过核糖体 RNA 基因内转录间隔区（ITS）区域的序列分析进行鉴定。虽然有 9 株菌在门水平上无法归属，但至少有 44 株菌属于子囊菌门（93%）、担子菌门（6%）和接合菌门（1%）15 个目、29 个属。随后的检测显示，19%的内生提取物对至少一种病原菌或真菌具有抗菌活性。采用琼脂扩散法测定抗菌活性，其中 4 株分离株的提取物活性最为显著。此外，高效液相色谱（HPLC）和超高效液相色谱-四极杆飞行时间质谱联用（UPLC-QTOF MS）分析表明，两种镰刀菌菌株中存在鬼臼毒素，其中镰刀菌（WB5121）的产量最高，为 277μg/g。综上所述，本研究数据表明，八角莲的各种内生真菌可能被开发为新型天然抗菌或抗癌药物的来源。
