Cunha Verusca Najara, de Paula Lima Mérica, Motta-Santos Daisy, Pesquero Jorge Luiz, de Andrade Rosangela Vieira, de Almeida Jeeser Alves, Araujo Ronaldo Carvalho, Grubert Campbell Carmen Silvia, Lewis John E, Simões Herbert Gustavo
Graduate Program on Physical Education and Health, Catholic University of Brasilia, Brasília, DF, Brazil.
Department of Physiology and Biophysics, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil.
Cell Biochem Funct. 2015 Oct;33(7):435-42. doi: 10.1002/cbf.3128. Epub 2015 Oct 14.
Type 2 diabetes mellitus (T2D) results in several metabolic and cardiovascular dysfunctions, clinically characterized by hyperglycaemia due to lower glucose uptake and oxidation. Physical exercise is an effective intervention for glycaemic control. However, the effects of exercising at different intensities have not yet been addressed. The present study analysed the effects of 8 weeks of training performed at different exercise intensities on type 4 glucose transporters (GLUT4) content and glycaemic control of T2D (ob/ob) and non-diabetic mice (ob/OB). The animals were divided into six groups, with four groups being subjected either to low-intensity (ob/obL and ob/OBL: 3% body weight, three times/week/40 min) or high-intensity (ob/obH and ob/OBH: 6% body weight, three times per week per 20 min) swimming training. An incremental swimming test was performed to measure aerobic fitness. After the training intervention period, glycaemia and the content of GLUT4 were quantified. Although both training intensities were beneficial, the high-intensity regimen induced a more significant improvement in GLUT4 levels and glycaemic profile compared with sedentary controls (p < 0.05). Only animals in the high-intensity exercise group improved aerobic fitness. Thus, our study shows that high-intensity training was more effective for increasing GLUT4 content and glycaemia reduction in insulin-resistant mice, perhaps because of a higher metabolic demand imposed by this form of exercise.
2型糖尿病（T2D）会导致多种代谢和心血管功能障碍，其临床特征为因葡萄糖摄取和氧化降低而出现高血糖。体育锻炼是控制血糖的有效干预措施。然而，不同强度运动的效果尚未得到研究。本研究分析了8周不同强度运动训练对T2D（ob/ob）和非糖尿病小鼠（ob/OB）的4型葡萄糖转运蛋白（GLUT4）含量及血糖控制的影响。将动物分为六组，其中四组分别进行低强度（ob/obL和ob/OBL：体重的3%，每周三次/每次40分钟）或高强度（ob/obH和ob/OBH：体重的6%，每周三次/每次20分钟）游泳训练。进行递增游泳测试以测量有氧适能。在训练干预期后，对血糖和GLUT4含量进行定量分析。尽管两种训练强度均有益处，但与久坐对照组相比，高强度训练方案能更显著地改善GLUT4水平和血糖状况（p < 0.05）。只有高强度运动组的动物有氧适能得到改善。因此，我们的研究表明，高强度训练对于增加胰岛素抵抗小鼠的GLUT4含量和降低血糖更为有效，这可能是由于这种运动形式带来了更高的代谢需求。
