Saat R E, Heineman E, de Bruin R W, Marquet R L, Jeekel J
Laboratory for Experimental Surgery, Erasmus University Rotterdam, The Netherlands.
Transplantation. 1989 Mar;47(3):451-3.
The effect of pretransplant specific blood transfusions and irradiation of the donor were studied in total orthotopic small bowel transplantation in rats, with the aim of ameliorating the graft-versus-host reaction. Syngeneic transplantation with or without irradiation of the donor with 10 Gy showed that small bowel transplantation is compatible with a normal nutritional status, when no rejection is involved. Transplantation in the histoincompatible WAG-to-BN combination without pretreatment resulted in rejection of the small bowel grafts in 16.6 +/- 2.7 days. Pretreatment with 3 BN blood transfusions to the WAG donor did not prolong the survival time; surprisingly, the transfusions induced a more-severe GVH reaction. Irradiation of the donor with 10 Gy was very effective in ameliorating the GVH disease, but the absence of the immunosuppressive effect of the GVH disease did lead to an accelerated graft rejection (7.5 +/- 0.9 days).
为了改善移植物抗宿主反应，在大鼠全原位小肠移植中研究了移植前特异性输血和供体照射的效果。对供体进行或不进行10 Gy照射的同基因移植表明，在不发生排斥反应时，小肠移植与正常营养状态相容。在组织不相容的WAG到BN组合中未经预处理进行移植，小肠移植物在16.6±2.7天发生排斥。对WAG供体进行3次BN输血预处理并未延长存活时间；令人惊讶的是，输血诱导了更严重的移植物抗宿主反应。对供体进行10 Gy照射在改善移植物抗宿主病方面非常有效，但移植物抗宿主病免疫抑制作用的缺失确实导致了移植排斥加速（7.5±0.9天）。
