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用于DNA微阵列的鼓泡法对胺基玻璃平台的改进。

Improvement in the amine glass platform by bubbling method for a DNA microarray.

作者信息

Jee Seung Hyun, Kim Jong Won, Lee Ji Hyeong, Yoon Young Soo

机构信息

Department of Chemical and Biological Engineering, Gachon University, Seongnam, Gyeonggi, Republic of Korea.

Genomics Clinical Research Institute, LabGenomics Co., Ltd., Bundang-gu, Seongnam-si, Gyeonggi-do, Republic of Korea.

出版信息

Int J Nanomedicine. 2015 Oct 3;10(Spec Iss):273-80. doi: 10.2147/IJN.S88320. eCollection 2015.

DOI:10.2147/IJN.S88320
PMID:26468293
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4601307/
Abstract

A glass platform with high sensitivity for sexually transmitted diseases microarray is described here. An amino-silane-based self-assembled monolayer was coated on the surface of a glass platform using a novel bubbling method. The optimized surface of the glass platform had highly uniform surface modifications using this method, as well as improved hybridization properties with capture probes in the DNA microarray. On the basis of these results, the improved glass platform serves as a highly reliable and optimal material for the DNA microarray. Moreover, in this study, we demonstrated that our glass platform, manufactured by utilizing the bubbling method, had higher uniformity, shorter processing time, lower background signal, and higher spot signal than the platforms manufactured by the general dipping method. The DNA microarray manufactured with a glass platform prepared using bubbling method can be used as a clinical diagnostic tool.

摘要

本文描述了一种对性传播疾病微阵列具有高灵敏度的玻璃平台。使用一种新颖的鼓泡方法在玻璃平台表面涂覆基于氨基硅烷的自组装单分子层。采用该方法，玻璃平台的优化表面具有高度均匀的表面修饰，以及在DNA微阵列中与捕获探针改善的杂交特性。基于这些结果，改进后的玻璃平台可作为DNA微阵列的高度可靠且最佳的材料。此外，在本研究中，我们证明，通过鼓泡法制造的玻璃平台比通过常规浸渍法制造的平台具有更高的均匀性、更短的处理时间、更低的背景信号和更高的斑点信号。用鼓泡法制备的玻璃平台制造的DNA微阵列可作为临床诊断工具。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c27f/4601307/af22eb93f227/ijn-10-273Fig7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c27f/4601307/24c93d44956b/ijn-10-273Fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c27f/4601307/e41d31417a96/ijn-10-273Fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c27f/4601307/5cd62feb0779/ijn-10-273Fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c27f/4601307/81df77c15162/ijn-10-273Fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c27f/4601307/a8fd17922527/ijn-10-273Fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c27f/4601307/bfac5f1c4ed8/ijn-10-273Fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c27f/4601307/af22eb93f227/ijn-10-273Fig7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c27f/4601307/24c93d44956b/ijn-10-273Fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c27f/4601307/e41d31417a96/ijn-10-273Fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c27f/4601307/5cd62feb0779/ijn-10-273Fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c27f/4601307/81df77c15162/ijn-10-273Fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c27f/4601307/a8fd17922527/ijn-10-273Fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c27f/4601307/bfac5f1c4ed8/ijn-10-273Fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c27f/4601307/af22eb93f227/ijn-10-273Fig7.jpg

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本文引用的文献

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A comparison of oligonucleotide-based microarray and real-time PCR for the detection of sexually transmitted infections.基于寡核苷酸的微阵列技术与实时荧光定量PCR技术在性传播感染检测中的比较
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