伊朗东北部硬斑病皮损中伯氏疏螺旋体的聚合酶链反应检测频率
Frequency of Borrelia in Morphea Lesion by Polymerase Chain Reaction in Northeast of Iran.
作者信息
Yazdanpanah Mohhamad Javad, Sharifi Norieh, Khooei Alireza, Banihashemi Mahnaz, Khaje-Daluee Mohammad, Shamsi Azadeh, Ghazvini Kiarash
机构信息
Department of Dermatology, Research Center for Skin Diseases and Cutaneous Leishmaniasis, Ghaem Hospital, Mashhad University of Medical Sciences, Mashhad, IR Iran.
Department of Pathology, Mashhad University of Medical Sciences, Mashhad, IR Iran.
出版信息
Jundishapur J Microbiol. 2015 Aug 27;8(8):e19730. doi: 10.5812/jjm.19730v2. eCollection 2015 Aug.
BACKGROUND
The etiology of morphea is still unknown. Borrelia spp. as a causative agent of morphea has been discussed since 1985, but the relationship remains uncertain.
OBJECTIVES
We aimed to find the frequency of Borrelia in morphea lesions by polymerase chain reaction (PCR) in northeast of Iran.
PATIENTS AND METHODS
Sixty six patients with morphea were prospectively included in the present study. For each patient, formalin-fixed, paraffin-embedded tissue blocks of skin lesion biopsies were examined for Borrelia spp. DNA using PCR.
RESULTS
No Borrelia DNA was detected by PCR in skin lesions of patients with morphea.
CONCLUSIONS
The result of this study showed no relationship between Borrelia infection and morphea lesions and in other word indicated that morphea, at least in Iran, is not caused by Borrelia spp.
背景
硬斑病的病因仍不清楚。自1985年以来，伯氏疏螺旋体作为硬斑病的病原体一直被讨论，但两者的关系仍不确定。
目的
我们旨在通过聚合酶链反应（PCR）检测伊朗东北部硬斑病皮损中伯氏疏螺旋体的检出率。
患者与方法
本研究前瞻性纳入了66例硬斑病患者。对每位患者，采用PCR检测福尔马林固定、石蜡包埋的皮肤病变活检组织块中的伯氏疏螺旋体DNA。
结果
硬斑病患者的皮肤病变经PCR检测未发现伯氏疏螺旋体DNA。
结论
本研究结果表明伯氏疏螺旋体感染与硬斑病皮损之间无关联，换句话说，至少在伊朗，硬斑病不是由伯氏疏螺旋体引起的。
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