Food and Nutritional Sciences Programme, School of Life Sciences, The Chinese University of Hong Kong , Shatin, New Territories (NT), Hong Kong Special Administrative Region (SAR), People's Republic of China.
J Agric Food Chem. 2015 Nov 4;63(43):9520-7. doi: 10.1021/acs.jafc.5b03482. Epub 2015 Oct 26.
Human respiratory syncytial virus (RSV) is a common pathogen that causes pneumonia and bronchiolitis in infants and young children. Our previous study showed that tangeretin from Citrus reticulate possessed potent in vitro anti-RSV effects comparable to that of ribavirin. Therefore, in this study, we investigated the in vivo anti-RSV activity of tangeretin in 3-week-old male BALB/c mice. A plaque reduction assay and fluorescence quantitative polymerase chain reaction (FQ-PCR) showed that tangeretin inhibited RSV replication in the lung of mice. Moreover, a luminex assay indicated tangeretin relieved RSV-induced lung inflammation by attenuating interleukin (IL)-1β secretion. Possible anti-inflammatory mechanisms of tangeretin were preliminarily explored using a RSV-infected macrophage model. A FQ-PCR, enzyme-linked immunosorbent assay (ELISA), and luciferase assay revealed that tangeretin inhibited RSV-induced inflammation by suppressing nuclear factor-κB (NF-κB) activation. This study demonstrates that tangeretin inhibited RSV replication and RSV-induced lung inflammation in vivo and may be useful in preventing and treating RSV infections and inflammation.
人呼吸道合胞病毒（RSV）是一种常见的病原体，可导致婴儿和幼儿患肺炎和细支气管炎。我们之前的研究表明，橘红中的川陈皮素有很强的体外抗 RSV 作用，可与利巴韦林相媲美。因此，在这项研究中，我们在 3 周龄雄性 BALB/c 小鼠中研究了川陈皮素的体内抗 RSV 活性。噬斑减少试验和荧光定量聚合酶链反应（FQ-PCR）表明川陈皮素抑制了小鼠肺部的 RSV 复制。此外，Luminex 检测表明，川陈皮素通过减轻白细胞介素（IL）-1β的分泌缓解 RSV 引起的肺部炎症。使用 RSV 感染的巨噬细胞模型初步探讨了川陈皮素的可能抗炎机制。FQ-PCR、酶联免疫吸附测定（ELISA）和荧光素酶测定显示，川陈皮素通过抑制核因子-κB（NF-κB）激活来抑制 RSV 诱导的炎症。本研究表明，川陈皮素抑制 RSV 复制和 RSV 诱导的肺部炎症，可能有助于预防和治疗 RSV 感染和炎症。
