Zhang Fei, Yu Jialin, Johnston Christopher R, Wang Yanqiu, Zhu Kai, Lu Feng, Zhang Zhipeng, Zou Jianqiu
Department of Innovation Center, Liaoning Academy of Agricultural Sciences, Shenyang, Liaoning, China.
Department of Crop and Soil Sciences, University of Georgia, Griffin, Georgia, United States.
PLoS One. 2015 Oct 15;10(10):e0140620. doi: 10.1371/journal.pone.0140620. eCollection 2015.
Osmopriming with PEG has potential to improve seed germination, seedling emergence, and establishment, especially under stress conditions. This research investigated germination performance, seedling establishment, and effects of osmopriming with PEG on physiology in sorghum seedlings and their association with post-priming stress tolerance under various soil moisture stress conditions. Results showed that seed priming increased the environmental range suitable for sorghum germination and has potential to provide more uniform and synchronous emergence. Physiologically, seed priming strengthened the antioxidant activities of APX, CAT, POD, and SOD, as well as compatible solutes including free amino acid, reducing sugar, proline, soluble sugar, and soluble protein contents. As a result, seed priming reduced lipid peroxidation and stabilized the cell membrane, resulting in increased stress tolerance under drought or excessive soil moisture environments. Overall, results suggested that seed priming with PEG was effective in improving seed germination and seedling establishment of sorghum under adverse soil moisture conditions. Osmopriming effectively strengthened the antioxidant system and increased osmotic adjustment, likely resulting in increased stress tolerance.
聚乙二醇渗调引发有潜力改善种子萌发、幼苗出土及定植情况，尤其是在胁迫条件下。本研究调查了在各种土壤水分胁迫条件下，聚乙二醇渗调引发对高粱种子萌发性能、幼苗定植的影响以及对高粱幼苗生理的影响，及其与引发后胁迫耐受性的关联。结果表明，种子引发扩大了适合高粱种子萌发的环境范围，并有潜力使种子出土更均匀、更同步。从生理角度来看，种子引发增强了抗坏血酸过氧化物酶（APX）、过氧化氢酶（CAT）、过氧化物酶（POD）和超氧化物歧化酶（SOD）的抗氧化活性，以及包括游离氨基酸、还原糖、脯氨酸、可溶性糖和可溶性蛋白含量在内的相容性溶质。因此，种子引发减少了脂质过氧化并稳定了细胞膜，从而在干旱或土壤水分过多的环境下提高了胁迫耐受性。总体而言，结果表明聚乙二醇种子引发能有效改善高粱在不利土壤水分条件下的种子萌发和幼苗定植情况。渗调引发有效地增强了抗氧化系统并增加了渗透调节能力，可能由此提高了胁迫耐受性。
