College of Agronomy, Shenyang Agricultural University, Shenyang, Liaoning, China.
Institute of Economic Crop, Shanxi Academy of Agricultural Sciences, Fenyang, Shanxi, China.
PLoS One. 2021 Jan 19;16(1):e0245505. doi: 10.1371/journal.pone.0245505. eCollection 2021.
Sorghum [Sorghum bicolor (L.) Moench] seed germination is sensitive to salinity, and seed priming is an effective method for alleviating the negative effects of salt stress on seed germination. However, few studies have compared the effects of different priming agents on sorghum germination under salt stress. In this study, we quantified the effects of priming with distilled water (HP), sodium chloride (NaCl), potassium chloride (KCl), calcium chloride (CaCl2), and polyethylene glycol (PEG) on sorghum seed germination under 150 mM NaCl stress. The germination potential, germination rate, germination index, vigor index, root length, shoot length, root fresh weight, shoot fresh weight, root dry weight, and shoot dry weight were significantly reduced by salt stress. Different priming treatments alleviated the germination inhibition caused by salt stress to varying degrees, and 50 mM CaCl2 was the most effective treatment. In addition, the mitigation effect of priming was stronger on root traits than on shoot traits. Mitigation efficacy was closely related to both the type of agent and the concentration of the solution. Principal component analysis showed that all concentrations of CaCl2 had higher scores and were clearly distinguished from other treatments based on their positive effects on all germination traits. The effects of the other agents varied with concentration. The priming treatments were divided into three categories based on their priming efficacy, and the 50, 100, and 150 mM CaCl2 treatments were placed in the first category. The 150 mM KCl, 10% PEG, HP, 150 mM NaCl, 30% PEG, and 50 mM KCl treatments were placed in the second category, and the 100 mM NaCl, 100 mM KCl, 20% PEG, and 50 mM NaCl treatments were least effective and were placed in the third category. Choosing appropriate priming agents and methods for future research and applications can ensure that crop seeds germinate healthily under saline conditions.
高粱[高粱（高粱）莫亨]种子发芽对盐度敏感，种子引发是缓解盐胁迫对种子发芽不利影响的有效方法。然而，很少有研究比较不同引发剂在盐胁迫下对高粱种子发芽的影响。在这项研究中，我们量化了用蒸馏水（HP）、氯化钠（NaCl）、氯化钾（KCl）、氯化钙（CaCl2）和聚乙二醇（PEG）引发对 150 mM NaCl 胁迫下高粱种子发芽的影响。盐胁迫显著降低了发芽势、发芽率、发芽指数、活力指数、根长、茎长、根鲜重、茎鲜重、根干重和茎干重。不同的引发处理在不同程度上缓解了盐胁迫对发芽的抑制作用，50 mM CaCl2 是最有效的处理。此外，引发处理对根性状的缓解作用强于对茎性状的缓解作用。缓解效果与试剂类型和溶液浓度密切相关。主成分分析表明，所有浓度的 CaCl2 得分较高，根据其对所有发芽性状的积极影响，与其他处理明显区分开来。其他试剂的效果随浓度而变化。根据引发效果将引发处理分为三类，将 50、100 和 150 mM CaCl2 处理置于第一类，将 150 mM KCl、10%PEG、HP、150 mM NaCl、30%PEG 和 50 mM KCl 处理置于第二类，将 100 mM NaCl、100 mM KCl、20%PEG 和 50 mM NaCl 处理置于第三类。在未来的研究和应用中选择合适的引发剂和方法可以确保作物种子在盐渍条件下健康发芽。
