School of Electrical and Electronic Engineering, Yonsei University , Seoul 120-749, Republic of Korea.
Department of Physics, Gakushuin University , Tokyo 171-8588, Japan.
ACS Nano. 2015 Nov 24;9(11):10896-908. doi: 10.1021/acsnano.5b03934. Epub 2015 Oct 20.
In this paper, we describe super-resolved sampling of live bacteria based on extraordinary optical transmission (EOT) of light. EOT is produced by surface plasmon confinement and coupling with nanostructures. Bacterial fluorescence is excited by the localized fields for subdiffraction-limited sampling. The concept was applied to elucidating bacterial dynamics of gliding Mycoplasma mobile (M. mobile). The results analyzed with multiple M. mobile bacteria show individual characters and reveal that M. mobile undergoes a significant axial variation at 94 nm. The sampling error of the method is estimated to be much smaller than 1/10 of the diffraction limit both in the lateral and depth axis. The method provides a powerful tool for investigation of biomolecular dynamics at subwavelength precision.
本文描述了基于光的超常光学传输（EOT）对活细菌进行超分辨采样的方法。EOT 是由表面等离激元的限制和与纳米结构的耦合产生的。细菌荧光通过局域场被激发，从而实现亚衍射极限的采样。该概念被应用于阐明滑行支原体（M. mobile）的细菌动力学。对多个 M. mobile 细菌进行分析的结果表明，每个细菌都具有独特的特征，并且揭示了 M. mobile 在 94nm 处经历了显著的轴向变化。该方法的采样误差估计在横向和深度轴上都小于衍射极限的 1/10。该方法为在亚波长精度下研究生物分子动力学提供了一种强大的工具。
