Pratt R E, Zou W M, Naftilan A J, Ingelfinger J R, Dzau V J
Division of Vascular Medicine and Atherosclerosis, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts 02115.
Am J Physiol. 1989 Mar;256(3 Pt 2):F469-74. doi: 10.1152/ajprenal.1989.256.3.F469.
The expression of the intrarenal renin angiotensin mRNAs in Wistar-Kyoto rats (WKY) and spontaneously hypertensive rats (SHR) were investigated in rats fed a low (0.02%)- or normal (1.6%)-sodium diet for 5 days. Total RNA was isolated from the kidneys and analyzed by both Northern and slot blots. The results indicated that the kidneys of all four groups of rats containing readibly detectable levels of renin and angiotensinogen mRNAs. The kidneys of the WKY contained higher levels of angiotensinogen mRNA under normal-salt diet compared with the SHR (P less than 0.01). Mild sodium depletion stimulated angiotensinogen mRNA in WKY kidneys by almost 50% compared with normal salt diet (P less than 0.01). In contrast, there was no significant difference in the renal angiotensinogen mRNA levels in the kidneys of SHR fed a low- or normal-sodium diet. Renin mRNA concentrations were comparable in both strains. Mild sodium depletion failed to yield any detectable changes in renin mRNA levels in either strain. Thus the SHR exhibits an alteration in the sodium regulation of intrarenal angiotensinogen mRNA expression. These results may have implications in the renal physiology of these animals.
在喂食低钠（0.02%）或正常钠（1.6%）饮食5天的Wistar-Kyoto大鼠（WKY）和自发性高血压大鼠（SHR）中，研究了肾内肾素血管紧张素mRNA的表达。从肾脏中分离出总RNA，并通过Northern印迹法和狭缝印迹法进行分析。结果表明，所有四组大鼠的肾脏中均可检测到肾素和血管紧张素原mRNA的水平。与SHR相比，正常盐饮食下WKY大鼠的肾脏中血管紧张素原mRNA水平更高（P<0.01）。与正常盐饮食相比，轻度钠缺乏使WKY大鼠肾脏中的血管紧张素原mRNA增加了近50%（P<0.01）。相反，喂食低钠或正常钠饮食的SHR大鼠肾脏中肾血管紧张素原mRNA水平没有显著差异。两种品系的肾素mRNA浓度相当。轻度钠缺乏未能使任一品系的肾素mRNA水平产生任何可检测到的变化。因此，SHR在肾内血管紧张素原mRNA表达的钠调节方面表现出改变。这些结果可能对这些动物的肾脏生理学有影响。
