Tropea Alessia, Wilson David, Cicero Nicola, Potortì Angela G, La Torre Giovanna L, Dugo Giacomo, Richardson David, Waldron Keith W
a Scienze dell'Ambiente, della Sicurezza, del Territorio, degli Alimenti e della Salute Department , University of Messina , Messina , Italy.
b Institute of Food Research , Norwich Research Park , Norwich , UK.
Nat Prod Res. 2016;30(9):1009-16. doi: 10.1080/14786419.2015.1095748. Epub 2015 Oct 15.
Ethanol production by fermentation is strongly dependent on media composition. Specific nutrients, such as trace elements, vitamins and nitrogen will affect the physiological state and, consequently, the fermentation performance of the micro-organism employed. The purpose of this study has been to assess the highest ethanol production by a minimal medium, instead of the more complex nutrients supplementation used during alcoholic fermentation. All fermentation tests were carried out using a microwell plate reader to monitor the processes. Two Saccharomyces cerevisiae strains (NCYC 2826 and NCYC 3445) were tested using three nitrogen sources, supplied with different vitamin and salts. The results show that solutions made of urea phosphate, KCl, MgSO4·7H2O, Ca-panthothenate, biotin allowed an ethanol yield of 22.9 and 23.4 g/L for strain NCYC 2826 and NCYC 3445, respectively, representing 90 and 92% of the theoretical yield. All tests were carried out using glucose as common reference carbon source.
通过发酵生产乙醇强烈依赖于培养基的组成。特定的营养物质，如微量元素、维生素和氮，会影响微生物的生理状态，进而影响所使用微生物的发酵性能。本研究的目的是评估使用基本培养基而非酒精发酵过程中使用的更复杂营养补充剂时的最高乙醇产量。所有发酵试验均使用微孔板读数器来监测过程。使用两种酿酒酵母菌株（NCYC 2826和NCYC 3445），采用三种氮源，并添加不同的维生素和盐。结果表明，由磷酸脲、氯化钾、七水硫酸镁、泛酸钙、生物素制成的溶液，对于菌株NCYC 2826和NCYC 3445，乙醇产量分别为22.9克/升和23.4克/升，分别占理论产量的90%和92%。所有试验均使用葡萄糖作为通用的参考碳源。
