人血清中备解素的多种沉降种类以及纯化备解素与补体第三成分的相互作用。
Multiple sedimenting species of properdin in human serum and interaction of purified properdin with the third component of complement.
作者信息
Chapitis J, Lepow I H
出版信息
J Exp Med. 1976 Feb 1;143(2):241-57. doi: 10.1084/jem.143.2.241.
Abstract
Normal human serum subjected to sucrose density gradient analysis exhibited multiple sedimenting species of properdin antigen. Properdin antigen distribution was dependent on serum concentration, ionic strength, temperature, and the presence of C3, and was not dependent on the presence of divalent metal cations or blood coagulation. In mixtures of purified components, properdin sedimented heavier in the presence of C3, C3b, or C3c. Addition of factor B to mixtures containing C3 and properdin was without effect. These data provide insights into earlier discrepancies concerning the sedimentation behavior of partially purified properdin, indicate a propensity of some constituents of the alternative pathway to form protein-protein complexes, and suggest caution in interpretation of immunopathological studies in which properdin deposits are found in the presence of C3.
摘要
对正常人血清进行蔗糖密度梯度分析时，发现备解素抗原存在多种沉降成分。备解素抗原的分布取决于血清浓度、离子强度、温度以及C3的存在，而不依赖于二价金属阳离子的存在或血液凝固。在纯化成分的混合物中，备解素在C3、C3b或C3c存在时沉降得更重。向含有C3和备解素的混合物中添加B因子没有效果。这些数据为早期关于部分纯化备解素沉降行为的差异提供了见解，表明替代途径的一些成分倾向于形成蛋白质-蛋白质复合物，并提示在解释在C3存在下发现备解素沉积的免疫病理学研究时应谨慎。
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