Frank Daniel L, Kurtz Ryan, Tinsley Nicholas A, Gassmann Aaron J, Meinke Lance J, Moellenbeck Daniel, Gray Michael E, Bledsoe Larry W, Krupke Christian H, Estes Ronald E, Weber Patrick, Hibbard Bruce E
USDA-ARS, Plant Genetics Research Unit, 205 Curtis Hall, University of Missouri, Columbia, MO 65211, USA Present address: Extension Service, Agriculture and Natural Resources Unit, West Virginia University, Morgantown, WV 26506, USA.
Syngenta Biotechnology, Inc., 3054 E. Cornwallis Rd., Research Triangle Park, NC 27709, USA Present address: Cotton Incorporated, 6399 Weston Pkwy, Cary, NC 27513, USA.
J Econ Entomol. 2015 Jun;108(3):1260-70. doi: 10.1093/jee/tov081. Epub 2015 Apr 24.
Seed blends containing various ratios of transgenic Bt maize (Zea mays L.) expressing the mCry3A+eCry3.1Ab proteins and non-Bt maize (near-isoline maize) were deployed alone and in combination with a soil applied pyrethroid insecticide (Force CS) to evaluate the emergence of the western corn rootworm, Diabrotica virgifera virgifera LeConte, in a total of nine field environments across the Midwestern United States in 2010 and 2011. Northern corn rootworm, Diabrotica barberi Smith & Lawrence emergence was also evaluated in four of these environments. Both western and northern corn rootworm beetle emergence from all Bt treatments was significantly reduced when compared with beetle emergence from near-isoline treatments. Averaged across all environments, western corn rootworm beetle emergence from 95:5, 90:10, and 80:20 seed blend ratios of mCry3A+eCry3.1Ab: near-isoline were 2.6-, 4.2-, and 6.7-fold greater than that from the 100:0 ratio treatment. Northern corn rootworm emergence from the same seed blend treatments resulted in 2.8-, 3.2-, and 4.2-fold more beetles than from the 100:0 treatment. The addition of Force CS (tefluthrin) significantly reduced western corn rootworm beetle emergence for each of the three treatments to which it was applied. Force CS also significantly delayed the number of days to 50% beetle emergence in western corn rootworms. Time to 50% beetle emergence in the 100% mCry3A+eCry3.1Ab treatment with Force CS was delayed 13.7 d when compared with western corn rootworm beetle emergence on near-isoline corn. These data are discussed in terms of rootworm resistance management.
含有不同比例表达mCry3A+eCry3.1Ab蛋白的转基因Bt玉米（Zea mays L.）和非Bt玉米（近等基因系玉米）的种子混合物，分别单独使用以及与土壤施用的拟除虫菊酯杀虫剂（Force CS）联合使用，以评估2010年和2011年美国中西部总共9个田间环境中西部玉米根虫（Diabrotica virgifera virgifera LeConte）的羽化情况。在其中4个环境中还评估了北部玉米根虫（Diabrotica barberi Smith & Lawrence）的羽化情况。与近等基因系处理相比，所有Bt处理的西部和北部玉米根虫甲虫羽化率均显著降低。在所有环境中平均来看，mCry3A+eCry3.1Ab与近等基因系种子混合比例为95:5、90:10和80:20时，西部玉米根虫甲虫羽化率分别比100:0比例处理高2.6倍、4.2倍和6.7倍。相同种子混合处理下，北部玉米根虫羽化产生的甲虫数量比100:0处理多2.8倍、3.2倍和4.2倍。添加Force CS（七氟菊酯）显著降低了所施用的三种处理中西部玉米根虫甲虫的羽化率。Force CS还显著延迟了西部玉米根虫达到50%甲虫羽化的天数。与近等基因系玉米上西部玉米根虫甲虫羽化相比，施用Force CS的100% mCry3A+eCry3.1Ab处理达到50%甲虫羽化的时间延迟了13.7天。这些数据从根虫抗性管理方面进行了讨论。
