Syngenta Crop Protection, Product Safety, LLC, PO Box 12257, 9 Davis Drive, Durham, NC 27709, USA.
Syngenta Crop Protection AG, Regulatory Affairs, Schwarzwaldallee 215, Basel, CH-4058, Switzerland.
J Insect Sci. 2017 Jan 1;17(2). doi: 10.1093/jisesa/iex003.
A shift toward transgenic crops which produce combinations of insecticidal proteins has increased the interest (Syngenta Seeds, Inc., Minnetonka, MN) in studying the potential for interactions amongst those proteins. We present a general testing method which accommodates proteins with nonoverlapping spectrums of activity. Our sequential testing approach first investigates groups of the proteins with overlapping activity; e.g., proteins active against Lepidoptera or Coleoptera, respectively. The Colby method is used to test for interactions within each respective group. Subsequently, the mixture of proteins within each group is regarded as a single entity and tests for interactions between the groups (when combined) is conducted using analysis of variance. We illustrate the method using Cry1Ab, Vip3Aa20, and Cry1F (a mixture of proteins active against Lepidoptera), and mCry3A and eCry3.1Ab (a mixture of proteins active against Coleoptera). These insecticidal proteins are produced by Bt11 × MIR162 × TC1507 × MIR604 × 5307 maize. We detected no interactions between Cry1Ab, Vip3Aa20, and Cry1F in tests using larvae of two different lepidopteran species, and possible slight antagonism between mCry3A and eCry3.1Ab with a coleopteran test species. We detected no effect of (eCry3.1Ab + mCry3A) on the potency of (Cry1Ab + Vip3Aa20 + Cry1F) to lepidopteran larvae, and no effect of (Cry1Ab + Vip3Aa20 + Cry1F) on the potency of (mCry3A + eCry3.1Ab) to coleopteran larvae. We discuss implications of these results for characterization of Bt11 × MIR162 × TC1507 × MIR604 × 5307 maize, and the value of the method for characterizing other transgenic crops that produce several insecticidal proteins.
向生产杀虫蛋白组合的转基因作物的转变增加了人们对研究这些蛋白之间相互作用潜力的兴趣（先正达种子公司，明尼通卡，明尼苏达州）。我们提出了一种通用的测试方法，可适应具有非重叠活性谱的蛋白。我们的顺序测试方法首先研究具有重叠活性的蛋白组；例如，分别针对鳞翅目或鞘翅目活性的蛋白。科利方法用于测试每个组内的相互作用。随后，将每个组内的蛋白混合物视为单个实体，并使用方差分析进行组间（组合时）相互作用的测试。我们使用 Cry1Ab、Vip3Aa20 和 Cry1F（对鳞翅目有效的蛋白混合物）以及 mCry3A 和 eCry3.1Ab（对鞘翅目有效的蛋白混合物）来说明该方法。这些杀虫蛋白由 Bt11×MIR162×TC1507×MIR604×5307 玉米产生。我们在使用两种不同鳞翅目幼虫的测试中未检测到 Cry1Ab、Vip3Aa20 和 Cry1F 之间的相互作用，并且在与鞘翅目测试物种的可能轻微拮抗作用。我们检测到 mCry3A 和 eCry3.1Ab 对鞘翅目测试物种的活性没有影响，也检测到（eCry3.1Ab + mCry3A）对（Cry1Ab + Vip3Aa20 + Cry1F）对鳞翅目幼虫活性的影响，以及（Cry1Ab + Vip3Aa20 + Cry1F）对（mCry3A + eCry3.1Ab）对鞘翅目幼虫活性的影响。我们讨论了这些结果对 Bt11×MIR162×TC1507×MIR604×5307 玉米特性描述的意义，以及该方法对描述生产几种杀虫蛋白的其他转基因作物的价值。
