Bernardi Oderlei, Bernardi Daniel, Amado Douglas, Sousa Renan S, Fatoretto Julio, Medeiros Fernanda C L, Conville Jared, Burd Tony, Omoto Celso
Departamento de Entomologia e Acarologia, Escola Superior de Agricultura "Luiz de Queiroz" (ESALQ/USP), Av., Pádua Dias 11, Piracicaba, São Paulo 13418-900, Brazil.
Syngenta Crop Protection, Av. Nações Unidas 18001, São Paulo 04795-900, Brazil.
J Econ Entomol. 2015 Dec;108(6):2711-9. doi: 10.1093/jee/tov219. Epub 2015 Jul 27.
Transgenic Agrisure Viptera 3 corn that expresses Cry1Ab, Vip3Aa20, and EPSPS proteins and Agrisure Viptera expressing Vip3Aa20 are used for control of Spodoptera frugiperda (J.E. Smith) and Diatraea saccharalis (F.) in Brazil. To support a resistance management program, resistance risk assessment studies were conducted to characterize the dose expression of Vip3Aa20 protein and level of control against these species. The Vip3Aa20 expression in Agrisure Viptera 3 and Agrisure Viptera decreased from V6 to V10 stage of growth. However, Vip3Aa20 expression in Agrisure Viptera 3 at V6 and V10 stages was 13- and 16-fold greater than Cry1Ab, respectively. The Vip3Aa20 expression in lyophilized tissue of Agrisure Viptera 3 and Agrisure Viptera diluted 25-fold in an artificial diet caused complete larval mortality of S. frugiperda and D. saccharalis. In contrast, lyophilized tissue of Bt11 at the same dilution does not provide complete mortality of these species. Agrisure Viptera 3 and Agrisure Viptera also caused a high level of mortality against S. frugiperda and D. saccharalis. Moreover, 100% mortality was observed for S. frugiperda larvae (neonates through fifth-instar larvae) when fed in corn with the Vip trait technology. Viptera corn achieves a high level of control against S. frugiperda and D. saccharalis providing a high dose, which is an important determination to support the refuge strategy for an effective resistance management program.
表达Cry1Ab、Vip3Aa20和EPSPS蛋白的转基因“先正达Viptera 3”玉米以及表达Vip3Aa20的“先正达Viptera”玉米用于巴西防治草地贪夜蛾（J.E.史密斯）和甘蔗二点螟（F.）。为支持抗性管理计划，开展了抗性风险评估研究，以表征Vip3Aa20蛋白的剂量表达以及对这些物种的防治水平。“先正达Viptera 3”和“先正达Viptera”中Vip3Aa20的表达在生长的V6至V10阶段有所下降。然而，“先正达Viptera 3”在V6和V10阶段的Vip3Aa20表达分别比Cry1Ab高13倍和16倍。在人工饲料中稀释25倍的“先正达Viptera 3”和“先正达Viptera”冻干组织中的Vip3Aa20表达导致草地贪夜蛾和甘蔗二点螟幼虫全部死亡。相比之下，相同稀释度的Bt11冻干组织不能使这些物种全部死亡。“先正达Viptera 3”和“先正达Viptera”对草地贪夜蛾和甘蔗二点螟也造成了高死亡率。此外，用具有Vip性状技术的玉米喂养时，观察到草地贪夜蛾幼虫（从新生幼虫到五龄幼虫）100%死亡。Viptera玉米对草地贪夜蛾和甘蔗二点螟实现了高水平防治，提供了高剂量，这是支持有效抗性管理计划避难所策略的一项重要决定因素。
