Bernardi Oderlei, Bernardi Daniel, Horikoshi Renato J, Okuma Daniela M, Miraldo Leonardo L, Fatoretto Julio, Medeiros Fernanda Cl, Burd Tony, Omoto Celso
Department of Entomology and Acarology, University of São Paulo (ESALQ/USP), Piracicaba, São Paulo, Brazil.
Syngenta Crop Protection, São Paulo, Brazil.
Pest Manag Sci. 2016 Sep;72(9):1794-802. doi: 10.1002/ps.4223. Epub 2016 Feb 9.
Spodoptera frugiperda is one the main target pests of maize events expressing Vip3Aa20 protein from Bacillus thuringiensis (Bt) in Brazil. In this study, we selected a resistant strain of S. frugiperda on Bt maize expressing Vip3Aa20 protein and characterized the inheritance and fitness costs of the resistance.
The resistance ratio of the Vip3Aa20-resistant strain of S. frugiperda was >3200-fold. Neonates of the Vip3Aa20-resistant strain were able to survive and emerge as fertile adults on Vip3Aa20 maize, while larvae from susceptible and heterozygous strains did not survive. The inheritance of Vip3Aa20 resistance was autosomal recessive and monogenic. Life history studies to investigate fitness cost revealed an 11% reduction in the survival rate until adult stage and a ∼50% lower reproductive rate of the Vip3Aa20-resistant strain compared with susceptible and heterozygous strains.
This is the first characterization of S. frugiperda resistance to Vip3Aa protein. Our results provide useful information for resistance management programs designed to prevent or delay resistance evolution to Vip3Aa proteins in S. frugiperda. © 2016 Society of Chemical Industry.
草地贪夜蛾是巴西种植表达苏云金芽孢杆菌（Bt）Vip3Aa20蛋白的转基因玉米时的主要靶标害虫之一。在本研究中，我们筛选出了对表达Vip3Aa20蛋白的Bt玉米具有抗性的草地贪夜蛾品系，并对该抗性的遗传特性和适合度代价进行了表征。
草地贪夜蛾Vip3Aa20抗性品系的抗性倍数>3200倍。Vip3Aa20抗性品系的幼虫能够在Vip3Aa20玉米上存活并发育为可育成虫，而敏感品系和杂合品系的幼虫不能存活。Vip3Aa20抗性的遗传为常染色体隐性单基因遗传。研究适合度代价的生活史实验表明，与敏感品系和杂合品系相比，Vip3Aa20抗性品系直至成虫期的存活率降低了11%，繁殖率降低了约50%。
这是首次对草地贪夜蛾对Vip3Aa蛋白的抗性进行表征。我们的结果为旨在预防或延缓草地贪夜蛾对Vip3Aa蛋白产生抗性进化的抗性管理计划提供了有用信息。© 2016化学工业协会。
