Ageichenko A V, Kalutsky P V, Medvedeva O A, Korolev V A
Zh Mikrobiol Epidemiol Immunobiol. 2015 Jul-Aug(4):84-8.
Study the composition of colon microbiocenosis and antioxidant properties of colonocytes under the conditions of experimental dysbiosis and prophylaxis application of emoxipin.
With the aim of prophylaxis, antioxidant emoxipin was administered to mice, in which medicinal dysbiosis was formed by intraperitoneal administration of gentamicin. Quantitative and qualitative study of mucous microflora of the mice colon was carried out by a bacteriological method. The state of lipid peroxidation system was judged by the content of acylhydroperoxides and malonic dialdehyde, system of antioxidant protection--by enzyme activity (catalase and superoxide dismutase).
Experimental dysbiosis of the colon manifested by changes in the composition of mucous microflora of the animals and antioxidant properties of colonocytes. Dysbiosis prophylaxis by emoxipin resulted in normalization of the activity of antioxidant protection enzymes and products of lipid peroxidation in intestine tissue.
The study results allow to consider, that use of emoxipin results in an increase of adaptive-compensatory capabilities of the macroorganism during experimental dysbiosis.
研究实验性生态失调及应用艾莫昔平进行预防的条件下结肠微生物群落的组成及结肠细胞的抗氧化特性。
为进行预防，将抗氧化剂艾莫昔平给予小鼠，通过腹腔注射庆大霉素在小鼠体内形成药物性生态失调。采用细菌学方法对小鼠结肠黏液微生物群进行定量和定性研究。通过酰基氢过氧化物和丙二醛的含量判断脂质过氧化系统的状态，通过酶活性（过氧化氢酶和超氧化物歧化酶）判断抗氧化保护系统的状态。
结肠的实验性生态失调表现为动物黏液微生物群组成及结肠细胞抗氧化特性的改变。艾莫昔平预防生态失调可使肠组织中抗氧化保护酶的活性及脂质过氧化产物恢复正常。
研究结果表明，在实验性生态失调期间，使用艾莫昔平可提高机体的适应性代偿能力。
