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用于克雷伯菌属分离与鉴定的显色合成培养基“克雷伯菌5-ASK铬-C”

[THE CHROMOGENIC SYNTHETIC MEDIUM "KLEBSIELLA 5-ASK CHROM-C" FOR ISOLATION AND IDENTIFICATION OF KLEBSIELLAE].

作者信息

Sivolodskii E P

出版信息

Klin Lab Diagn. 2015 May;60(5):48-51.

Abstract

The chromogenic synthetic medium "Klebsiella 5-ASK CHROM-C was developedfor isolation and identification of klebsiellae of species of K. pneumoniae subsp. pneumoniae K. oxytoca, K. mobilis according chromogenic reaction to enzyme 5-aminosalycilate decarboxylase as a unique marker of genus Klebsiella. The L-proline and L-calcium glutamate are used as a source of nitrogen and carbon in medium. The consistency of composition of growth medium that ensure its regularity. The diagnostic sensitivity of chromogenic medium is 95.3 ± 1.7%; diagnostic specificity is 100%; analytical sensitivity is 1-2 colony-forming units per ml-1. The identification of Klebsiella is achieved simultaneously with their isolation during 24 = 48 hours. The test of 5-ASK decarboxylase using two chromogenic mediums "Klebsiella 5-ASK CHROM-C" permits identifying additionally K. pneumoniae subsp, ozaenae, K .pneumoniae subsp. Rhinoscleromatis.

摘要

产色合成培养基“克雷伯菌5-ASK CHROM-C”是为根据对5-氨基水杨酸脱羧酶的产色反应作为克雷伯菌属的独特标记,分离和鉴定肺炎克雷伯菌亚种肺炎克雷伯菌、产酸克雷伯菌、催娩克雷伯菌而开发的。L-脯氨酸和L-谷氨酸钙用作培养基中的氮源和碳源。生长培养基成分的一致性确保了其规律性。产色培养基的诊断敏感性为95.3±1.7%;诊断特异性为100%;分析敏感性为每毫升1-2个菌落形成单位。在24至48小时内分离克雷伯菌的同时即可完成其鉴定。使用两种产色培养基“克雷伯菌5-ASK CHROM-C”进行5-ASK脱羧酶检测可额外鉴定肺炎克雷伯菌亚种臭鼻亚种、肺炎克雷伯菌亚种鼻硬结亚种。

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