Faktor O, Toister-Achituv M, Kamensky B
Department of Entomology, Hebrew University of Jerusalem, Rehovot, Israel.
Virus Genes. 1995;11(1):47-52. doi: 10.1007/BF01701661.
The Spodoptera littoralis multicapsid nuclear polyhedrosis virus (SlMNPV) is a member of the Baculoviridae that shows a distant genetic relationship to the prototype Autographa californica MNPV (AcMNPV). Using an AcMNPV gene-specific probe, we identified and mapped an ecdysteroid UDP-glucosyltransferase (egt) gene in the genome of SlMNPV. Sequence determination of a part from the hybridizing DNA fragment revealed an open reading frame of 1548 nucleotides that exhibits 38% and 44% identity to the egt amino acid sequences of AcMNPV and Lymantria dispar MNPV (LdMNPV), respectively. Sequences flanking the SlMNPV egt gene, including the promoter region, were found to be unique to the virus. The presence of this nonstructural gene in SlMNPV and several other baculoviruses points to the importance of egt for the viral infection process.
滨海夜蛾多粒包埋核型多角体病毒（SlMNPV）是杆状病毒科的成员，与原型苜蓿银纹夜蛾多粒包埋核型多角体病毒（AcMNPV）存在较远的遗传关系。我们使用AcMNPV基因特异性探针，在SlMNPV基因组中鉴定并定位了一个蜕皮甾体UDP-葡萄糖基转移酶（egt）基因。对杂交DNA片段的一部分进行序列测定，发现一个1548个核苷酸的开放阅读框，其与AcMNPV和舞毒蛾多粒包埋核型多角体病毒（LdMNPV）的egt氨基酸序列分别具有38%和44%的同一性。发现SlMNPV egt基因两侧的序列，包括启动子区域，是该病毒所特有的。SlMNPV和其他几种杆状病毒中存在这个非结构基因，表明egt对病毒感染过程具有重要性。
