Jain Rajeev, Gupta Manoj Kumar, Chauhan Abhishek, Pandey Vivek, Reddy Mudiam Mohana Krishna
Analytical Chemistry Laboratory, Regulatory Toxicology Group, CSIR-Indian Institute of Toxicology Research, MG Marg, Lucknow 226001, India.
Present address: Central Forensic Science Laboratory, Directorate of Forensic Science Services, Ministry of Home Affairs, Govt of India, House no. 16, Lachit Bhorpukhan Path, Tetalia, Gotanagar, Guwahati (Assam) - 781033, India.
Bioanalysis. 2015 Oct;7(19):2451-9. doi: 10.4155/bio.15.162. Epub 2015 Oct 16.
Valproic acid (VPA) is an anticonvulsant drug used for the treatment of epilepsy and bipolar disorder. A method based on simultaneous derivatization and dispersive liquid-liquid microextraction followed by GC-MS/MS analysis has been developed for the determination of VPA in urine samples.
This optimized and validated method shows good linearity with R(2) value of 0.999. LOD and LOQ of VPA was found to be 0.4 ng ml(-1) and 1.4 ng ml(-1), respectively. Recovery of VPA was found to be in the range of 80 to 92%.
The developed method can find its wide applicability for the routine analysis of VPA in toxicological and clinical laboratories.
丙戊酸（VPA）是一种用于治疗癫痫和双相情感障碍的抗惊厥药物。已开发出一种基于同时衍生化和分散液液微萃取然后进行气相色谱-串联质谱分析的方法，用于测定尿液样本中的VPA。
这种经过优化和验证的方法显示出良好的线性，R(2)值为0.999。发现VPA的检测限和定量限分别为0.4 ng/ml和1.4 ng/ml。VPA的回收率在80%至92%范围内。
所开发的方法可在毒理学和临床实验室中广泛应用于VPA的常规分析。
