Jain Rajeev, Mudiam Mohana Krishna Reddy, Ch Ratnasekhar, Chauhan Abhishek, Khan Haider A, Murthy Rc
Analytical Chemistry Section, CSIR-Indian Institute of Toxicology Research, M G Marg, Lucknow, 226 001, UP, India.
Bioanalysis. 2013 Sep;5(18):2277-86. doi: 10.4155/bio.13.188.
Silylation is a widely used derivatization method for the analysis of polar analytes by GC-MS. Ultrasound-assisted dispersive liquid-liquid microextraction (UA-DLLME) is an ecofriendly, rapid and simple microextraction method. For the first time, a novel approach has been developed and applied for the analysis of quinine in urine by combining UA-DLLME with injection port silylation.
The LOD and LOQ were found to be 5.4 and 18 ng/ml. The intra- and inter-day precisions were less than 5 and 8%, respectively. Mean recoveries of quinine were found to be in the range of 87 to 96%.
Ultrasound-assisted dispersive liquid-liquid microextraction is rapid, simple and consumes less reagent for the analysis of polar analytes such as quinine.
硅烷化是一种广泛用于通过气相色谱-质谱联用（GC-MS）分析极性分析物的衍生化方法。超声辅助分散液液微萃取（UA-DLLME）是一种环保、快速且简单的微萃取方法。首次开发并应用了一种将UA-DLLME与进样口硅烷化相结合的新方法用于尿液中奎宁的分析。
检测限（LOD）和定量限（LOQ）分别为5.4和18 ng/ml。日内和日间精密度分别小于5%和8%。奎宁的平均回收率在87%至96%范围内。
超声辅助分散液液微萃取对于分析诸如奎宁等极性分析物而言快速、简单且试剂消耗少。
