1977年沃尔氏三斑芋螺线粒体全基因组的特征分析
Characterization of the complete mitochondrial genome of Conus tribblei Walls, 1977.
作者信息
Barghi Neda, Concepcion Gisela P, Olivera Baldomero M, Lluisma Arturo O
机构信息
a Marine Science Institute, University of the Philippines-Diliman , Quezon City , Philippines.
b Philippine Genome Center, University of the Philippines , Quezon City , Philippines , and.
出版信息
Mitochondrial DNA A DNA Mapp Seq Anal. 2016 Nov;27(6):4451-4452. doi: 10.3109/19401736.2015.1089566. Epub 2015 Oct 16.
The genus Conus sensu lato consists of 500-700 species. However, the mitochondrial genomes of only few species have been fully sequenced and reported so far. In this study, the complete mitochondrial genome of Conus tribblei, a member of the poorly known subgenus Splinoconus is sequenced with the mean coverage of 604×. The mitochondrial genome is 15 570 bp long and consists of genes encoding for 13 respiratory chain proteins, 22 tRNA and 2 rRNA. The gene organization is highly conserved among the Conus species. The longest intergenic region between tRNA-Phe and cytochrome c oxidase subunit III (cox3), which in C. tribblei is 169 bp long and contains a 112 bp long segment of inverted repeat, represents the putative control region. The control regions of Conus species exhibited variability in the length and position of the inverted repeats. Therefore, this region may have the potential to be used as a genetic marker for species discrimination.
广义芋螺属包含500 - 700个物种。然而，到目前为止，只有少数物种的线粒体基因组已被完全测序并报道。在本研究中，对鲜为人知的裂芋螺亚属成员特里布尔芋螺的完整线粒体基因组进行了测序，平均覆盖度为604×。该线粒体基因组长度为15570 bp，由编码13种呼吸链蛋白、22种tRNA和2种rRNA的基因组成。基因组织在芋螺物种中高度保守。tRNA - Phe和细胞色素c氧化酶亚基III（cox3）之间最长的基因间隔区，在特里布尔芋螺中长169 bp，包含一个112 bp长的反向重复序列片段，代表假定的控制区。芋螺物种的控制区在反向重复序列的长度和位置上表现出变异性。因此，该区域可能有潜力用作物种鉴别的遗传标记。
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