Sun Chuanzheng, Liu Xunyang, Yi Zhongjie, Xiao Xuefei, Yang Mingshi, Hu Gui, Liu Huaizheng, Liao Liangkan, Huang Feizhou
Emergency Department, the Third Xiangya Hospital of Central South University, Changsha, Hunan, China.
IUBMB Life. 2015 Nov;67(11):847-52. doi: 10.1002/iub.1442. Epub 2015 Oct 16.
Non-alcoholic fatty liver disease (NAFLD) is a common liver disorder poising burgeoning health problem to humans. Recent studies have shown that long non-coding RNA (lncRNA) plays critical roles in a myriad of biological processes and human diseases. Since the roles of lncRNA in NAFLD remain unknown, they were investigated in the study. Microarray expression profiling of mRNAs and lncRNAs was conducted using RNA extracted from patients with and without NAFLD. One thousand seven hundred thirty-five lncRNAs and 1485 mRNA were found differentially expressed in NAFLD samples compared with those in control samples. Among them 535 and 1,200 lncRNAs were upregulated and downregulated in NAFLD, respectively; 760 and 725 mRNAs were upregulated and downregulated in NAFLD, respectively. Moreover, seven lncRNAs and seven mRNAs that were highly up- or downregulated in NAFLD samples were validated by quantitative real-time polymer chain reactions. Kyoto Encyclopedia of Genes and Genomes pathway analysis and Gene Ontology analysis for the differentially expressed mRNAs showed that these RNAs are involved in various metabolic processes, cellular components, and molecular functions. Our findings indicate that the expression profiles of lncRNAs have changed in NAFLD as compared with normal liver, and the identified regulated RNAs may provide novel insight into the molecular mechanisms underlying the disease and potential novel diagnostic or therapeutic targets for NAFLD.
非酒精性脂肪性肝病（NAFLD）是一种常见的肝脏疾病，给人类健康带来日益严峻的问题。最近的研究表明，长链非编码RNA（lncRNA）在众多生物过程和人类疾病中发挥着关键作用。由于lncRNA在NAFLD中的作用尚不清楚，因此在本研究中对其进行了调查。使用从患有和未患有NAFLD的患者中提取的RNA进行mRNA和lncRNA的微阵列表达谱分析。与对照样品相比，在NAFLD样品中发现1735个lncRNA和1485个mRNA差异表达。其中，535个和1200个lncRNA在NAFLD中分别上调和下调；760个和725个mRNA在NAFLD中分别上调和下调。此外，通过定量实时聚合酶链反应验证了在NAFLD样品中高度上调或下调的7个lncRNA和7个mRNA。对差异表达的mRNA进行京都基因与基因组百科全书通路分析和基因本体分析表明，这些RNA参与了各种代谢过程、细胞成分和分子功能。我们的研究结果表明，与正常肝脏相比，NAFLD中lncRNA的表达谱发生了变化，所鉴定的受调控RNA可能为该疾病的分子机制提供新的见解，并为NAFLD提供潜在的新诊断或治疗靶点。
