聚乙烯醇降解菌株鞘氨醇单胞菌属（Sphingopyxis）sp. 113P3（NBRC 111507）的全基因组序列
Complete Genome Sequence of Polyvinyl Alcohol-Degrading Strain Sphingopyxis sp. 113P3 (NBRC 111507).
作者信息
Ohtsubo Yoshiyuki, Nagata Yuji, Numata Mitsuru, Tsuchikane Kieko, Hosoyama Akira, Yamazoe Atsushi, Tsuda Masataka, Fujita Nobuyuki, Kawai Fusako
机构信息
Department of Environmental Life Sciences, Graduate School of Life Sciences, Tohoku University, Sendai, Japan
Department of Environmental Life Sciences, Graduate School of Life Sciences, Tohoku University, Sendai, Japan.
出版信息
Genome Announc. 2015 Oct 15;3(5):e01169-15. doi: 10.1128/genomeA.01169-15.
Strain 113P3 was isolated from activated sludge and identified as a polyvinyl alcohol (PVA)-degrading Pseudomonas species; it was later reidentified as Sphingopyxis species. Only three genes are directly relevant to the metabolism of PVA and comprise the pva operon, which was deposited as accession no. AB190228. Here, we report the complete genome sequence of strain 113P3, which has been conserved as a stock culture (NBRC 111507) at the Biological Resource Center, National Institute of Technology and Evaluation (NITE) (Tokyo, Japan). The genome of strain 113P3 is composed of a 4.4-Mb circular chromosome and a 243-kb plasmid. The whole finishing was conducted in silico except for four PCRs. The sequence corresponding to AB190288 exists on the chromosome.
菌株113P3从活性污泥中分离出来，被鉴定为一种能降解聚乙烯醇（PVA）的假单胞菌属物种；后来它被重新鉴定为鞘氨醇单胞菌属物种。只有三个基因与PVA的代谢直接相关，它们组成了pva操纵子，该操纵子的登录号为AB190228。在此，我们报告菌株113P3的完整基因组序列，该菌株已作为保藏培养物（NBRC 111507）保存在日本国立技术与评价研究所（NITE）（东京，日本）的生物资源中心。菌株113P3的基因组由一条4.4 Mb的环状染色体和一个243 kb的质粒组成。除了四次PCR外，整个基因组的完成是在计算机上进行的。与AB190288对应的序列存在于染色体上。
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