Huang Wei, Kim Seong Jong, Liu Joyce, Zhang Wenjun
Department of Chemical and Biomolecular Engineering, University of California , Berkeley, California 94720, United States.
Org Lett. 2015 Nov 6;17(21):5344-7. doi: 10.1021/acs.orglett.5b02707. Epub 2015 Oct 16.
The cyclizidine biosynthetic gene cluster was identified from Streptomyces NCIB 11649, which revealed the polyketide biosynthetic machinery for cyclizidine alkaloid biosynthesis. Both in vivo mutagenesis study and in vitro biochemical analysis provided insight into the timing and mechanism of the biosynthetic enzymes that produce cyclizidine-type indolizidine alkaloids.
从链霉菌NCIB 11649中鉴定出了环嗪生物合成基因簇,该基因簇揭示了环嗪生物碱生物合成的聚酮生物合成机制。体内诱变研究和体外生化分析都为产生环嗪型吲哚里西啶生物碱的生物合成酶的作用时机和机制提供了深入了解。