拟南芥CDF2在初级微小RNA转录和转录后调控中的作用
The Roles of Arabidopsis CDF2 in Transcriptional and Posttranscriptional Regulation of Primary MicroRNAs.
作者信息
Sun Zhenfei, Guo Tongtong, Liu Yin, Liu Qi, Fang Yuda
机构信息
National Key Laboratory of Plant Molecular Genetics, Shanghai Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China.
出版信息
PLoS Genet. 2015 Oct 16;11(10):e1005598. doi: 10.1371/journal.pgen.1005598. eCollection 2015 Oct.
The precise regulation of microRNA (miRNA) transcription and processing is important for eukaryotic development. Plant miRNAs are first transcribed as stem-loop primary miRNAs (pri-miRNAs) by RNA polymerase II,then cleaved in the nucleus into mature miRNAs by Dicer-like 1 (DCL1). We identified a cycling DOF transcription factor, CDF2, which interacts with DCL1 and regulates the accumulation of a population of miRNAs. CDF2 binds directly to the promoters of some miRNAs and works as a transcription activator or repressor for these miRNA genes. CDF2 binds preferentially to the pri-miRNAs regulated by itself and affects DCL1-mediated processing of these pri-miRNAs. Genetically, CDF2 works in the same pathway as miR156 or miR172 to control flowering. We conclude that CDF2 regulates a group of pri-miRNAs at both the transcriptional and posttranscriptional levels to maintain proper levels of their mature miRNAs to control plant development.
微小RNA（miRNA）转录和加工的精确调控对真核生物发育至关重要。植物miRNA首先由RNA聚合酶II转录为茎环结构的初级miRNA（pri-miRNA），然后在细胞核中被类Dicer 1（DCL1）切割成成熟的miRNA。我们鉴定出一个周期性表达的DOF转录因子CDF2，它与DCL1相互作用并调控一群miRNA的积累。CDF2直接结合一些miRNA的启动子，并作为这些miRNA基因的转录激活因子或抑制因子发挥作用。CDF2优先结合其自身调控的pri-miRNA，并影响DCL1介导的这些pri-miRNA的加工过程。在遗传学上，CDF2与miR156或miR172在同一途径中发挥作用来控制开花。我们得出结论，CDF2在转录和转录后水平调控一组pri-miRNA，以维持其成熟miRNA的适当水平，从而控制植物发育。
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