Research Institute of Forestry, Chinese Academy of Forestry, Key Laboratory of Tree Breeding and Cultivation, State Forestry Administration, Beijing, China.
Nat Genet. 2013 Apr;45(4):456-61, 461e1-2. doi: 10.1038/ng.2569. Epub 2013 Feb 24.
Bamboo represents the only major lineage of grasses that is native to forests and is one of the most important non-timber forest products in the world. However, no species in the Bambusoideae subfamily has been sequenced. Here, we report a high-quality draft genome sequence of moso bamboo (P. heterocycla var. pubescens). The 2.05-Gb assembly covers 95% of the genomic region. Gene prediction modeling identified 31,987 genes, most of which are supported by cDNA and deep RNA sequencing data. Analyses of clustered gene families and gene collinearity show that bamboo underwent whole-genome duplication 7-12 million years ago. Identification of gene families that are key in cell wall biosynthesis suggests that the whole-genome duplication event generated more gene duplicates involved in bamboo shoot development. RNA sequencing analysis of bamboo flowering tissues suggests a potential connection between drought-responsive and flowering genes.
竹子是唯一原产于森林的大型禾本科植物，也是世界上最重要的非木材林产品之一。然而，尚未对任何一个竹亚科物种进行测序。在此，我们报告了毛竹（P. heterocycla var. pubescens）高质量的基因组草图序列。该 2.05-Gb 组装覆盖了基因组区域的 95%。通过基因预测建模，鉴定出 31987 个基因，其中大多数基因都得到了 cDNA 和深度 RNA 测序数据的支持。聚类基因家族和基因共线性分析表明，竹子在 700-1200 万年前经历了全基因组复制。对细胞壁生物合成关键基因家族的鉴定表明，全基因组复制事件产生了更多参与竹笋发育的基因重复。对竹子开花组织的 RNA 测序分析表明，干旱响应基因和开花基因之间可能存在联系。
