Tsu Brian V, Saier Milton H
Department of Molecular Biology, Division of Biological Sciences, University of California at San Diego, La Jolla, California, United States of America.
PLoS One. 2015 Oct 16;10(10):e0137184. doi: 10.1371/journal.pone.0137184. eCollection 2015.
The LysE superfamily consists of transmembrane transport proteins that catalyze export of amino acids, lipids and heavy metal ions. Statistical means were used to show that it includes newly identified families including transporters specific for (1) tellurium, (2) iron/lead, (3) manganese, (4) calcium, (5) nickel/cobalt, (6) amino acids, and (7) peptidoglycolipids as well as (8) one family of transmembrane electron carriers. Internal repeats and conserved motifs were identified, and multiple alignments, phylogenetic trees and average hydropathy, amphipathicity and similarity plots provided evidence that all members of the superfamily derived from a single common 3-TMS precursor peptide via intragenic duplication. Their common origin implies that they share common structural, mechanistic and functional attributes. The transporters of this superfamily play important roles in ionic homeostasis, cell envelope assembly, and protection from excessive cytoplasmic heavy metal/metabolite concentrations. They thus influence the physiology and pathogenesis of numerous microbes, being potential targets of drug action.
LysE超家族由催化氨基酸、脂质和重金属离子输出的跨膜转运蛋白组成。采用统计学方法表明，它包括新鉴定的家族，其中有对（1）碲、（2）铁/铅、（3）锰、（4）钙、（5）镍/钴、（6）氨基酸和（7）肽糖脂具有特异性的转运蛋白，以及（8）一个跨膜电子载体家族。鉴定出了内部重复序列和保守基序，多重比对、系统发育树以及平均亲水性、两亲性和相似性图谱提供了证据，表明该超家族的所有成员通过基因内重复从单一的共同3-TMS前体肽衍生而来。它们的共同起源意味着它们具有共同的结构、机制和功能属性。这个超家族的转运蛋白在离子稳态、细胞包膜组装以及防止细胞质中重金属/代谢物浓度过高方面发挥着重要作用。因此，它们影响众多微生物的生理和发病机制，是药物作用的潜在靶点。
