School of Plant Sciences, The University of Arizona, Tucson, AZ, USA.
Department of Entomology and Nematology, IFAS, Southwest Florida Research and Education Center, University of Florida, Immokalee, FL, USA.
Sci Rep. 2024 Jul 15;14(1):16248. doi: 10.1038/s41598-024-65156-4.
Psyllid species, including the potato psyllid (PoP) Bactericera cockerelli (Sulc) (Triozidae) serve as host and vector of "Candidatus Liberibacter spp." ("Ca. Liberibacter"), which also infects diverse plant hosts, including citrus and tomato. Psyllid transmission of "Ca. Liberibacter" is circulative and propagative. The time of "Ca. Liberibacter" acquisition and therefore vector life stage most competent for bacterial transmission varies by pathosystems. Here, the potato psyllid-"Ca. Liberibacter solanacearum" (CLso) pathosystem was investigated to dissect CLso-prophage interactions in the tomato plant and PoP-psyllid host by real-time quantitative reverse transcriptase amplification of CLso genes/loci with predicted involvement in host infection and psyllid-CLso transmission. Genes/loci analyzed were associated with (1) CLso-adhesion, -invasion, -pathogenicity, and -motility, (2) prophage-adhesion and pathogenicity, and (3) CLso-lysogenic cycle. Relative gene expression was quantified by qRT-PCR amplification from total RNA isolated from CLso-infected 1st-2nd and 4th-5th nymphs and teneral adults and CLso-infected tomato plants in which CLso infection is thought to occur without SC1-SC2 replication. Gene/loci expression was host-dependent and varied with the psyllid developmental stage. Loci previously associated with repressor-anti-repressor regulation in the "Ca Liberibacter asiaticus"-prophage pathosystem, which maintains the lysogenic cycle in Asian citrus psyllid Diaphorina citri, were expressed in CLso-infected psyllids but not in CLso-infected tomato plants.
榆叶跳甲，包括马铃薯跳甲（PoP）Bactericera cockerelli（Sulc）（Triozidae），是“Candidatus Liberibacter spp.”（“Ca. Liberibacter”）的宿主和载体，“Ca. Liberibacter”也感染多种植物宿主，包括柑橘和番茄。榆叶跳甲传播“Ca. Liberibacter”是循环和增殖的。“Ca. Liberibacter”的获得时间以及因此最适合细菌传播的载体生活阶段因病原系统而异。在这里，研究了马铃薯跳甲-“Ca. Liberibacter solanacearum”（CLso）病原系统，通过实时定量逆转录聚合酶链反应扩增与预测参与宿主感染和跳甲-CLso 传播有关的 CLso 基因/基因座，剖析 CLso 噬菌体与番茄植物和 PoP 跳甲宿主的相互作用。分析的基因/基因座与（1）CLso-黏附、-入侵、-致病性和-运动性、（2）噬菌体黏附和致病性、和（3）CLso-溶原性周期有关。通过从 CLso 感染的 1 龄至 2 龄和 4 龄至 5 龄若虫和刚羽化的成虫以及被认为没有 SC1-SC2 复制的 CLso 感染的番茄植物中分离的总 RNA 进行 qRT-PCR 扩增来定量相对基因表达。基因/基因座表达依赖于宿主，并随跳甲发育阶段而变化。先前与“Ca Liberibacter asiaticus”-噬菌体病原系统中的阻遏物-抗阻遏物调节相关的基因座，该系统在亚洲柑橘木虱 Diaphorina citri 中维持溶原性周期，在 CLso 感染的跳甲中表达，但在 CLso 感染的番茄植物中不表达。
