Deng Quanjun, Xie Liqun, Li Hua
Departments of Gastroenterology, Affiliated Hospital of Logistics University of the Chinese People's Armed Police Forces, Tianjin 300162, China.
Departments of Gastroenterology, Affiliated Hospital of Logistics University of the Chinese People's Armed Police Forces, Tianjin 300162, China.
Biochem Biophys Res Commun. 2015 Nov 27;467(4):921-7. doi: 10.1016/j.bbrc.2015.10.043. Epub 2015 Oct 20.
Recent studies have shown that miR-506 plays important roles in human cancer progression. However, little is known about the function of miR-506 in hepatocellular carcinoma (HCC). In this study, we found that miR-506 significantly inhibits HCC cell proliferation in vitro and tumorigenicity in vivo. Moreover, miR-506 induced G1/S cell cycle arrest and apoptosis in HCC cells. Rho-associated protein kinase 1(ROCK1) was identified as a novel target of miR-506; overexpression of ROCK1 reversed the suppressive effects of miR-506 in HCC cells. Additionally, ROCK1 was found up-regulated and inversely correlated with miR-506 in HCC tissues. Therefore, our findings collectively suggest that miR-506 acts as a tumor suppressor via regulation of ROCK1 expression and may thus be a promising therapeutic target for HCC.
最近的研究表明，miR-506在人类癌症进展中发挥重要作用。然而，关于miR-506在肝细胞癌（HCC）中的功能知之甚少。在本研究中，我们发现miR-506在体外显著抑制HCC细胞增殖，并在体内抑制肿瘤发生。此外，miR-506诱导HCC细胞发生G1/S期细胞周期阻滞和凋亡。Rho相关蛋白激酶1（ROCK1）被确定为miR-506的一个新靶点；ROCK1的过表达逆转了miR-506对HCC细胞的抑制作用。此外，在HCC组织中发现ROCK1上调且与miR-506呈负相关。因此，我们的研究结果共同表明，miR-506通过调节ROCK1表达发挥肿瘤抑制作用，因此可能是HCC的一个有前景的治疗靶点。
