Attah E B, Ani A N, Odor E I
Acta Cytol. 1977 Jan-Feb;21(1):151-3.
The spontaneous decay of methyl green-pyroninophilia in Burkitt's lymphoma was studied at four temperatures (0 degrees C to 56 degrees C) in four biopsies using Kurnick's method. The decay is temperature and time related and is presumably due to intrinsic enzymatic action, probably ribonuclease. Imprints from tumor tissue preserved at 0 degrees C lose pyroninophilia by six hours; from tumor preserved at room temperature pyroninophilia, in most instances, would have been lost by three hours, from tumor tissue maintained at 37 degrees C, by two hours in most instances and by 15 minutes at 56 degrees C in most instances. The absence of pyroninophilia after these intervals at the appropriate temperatures should not detract from the diagnosis of Burkitt's Lymphoma.
采用库尔尼克方法，在四个温度（0摄氏度至56摄氏度）下对四例活检组织中伯基特淋巴瘤甲基绿-派若宁嗜色性的自发衰变进行了研究。这种衰变与温度和时间相关，推测是由于内在的酶促作用，可能是核糖核酸酶。保存在0摄氏度的肿瘤组织印记在6小时后失去派若宁嗜色性；保存在室温下的肿瘤，在大多数情况下，3小时后派若宁嗜色性会消失；保存在37摄氏度的肿瘤组织，在大多数情况下2小时后消失，在56摄氏度时大多数情况下15分钟后消失。在适当温度下经过这些时间间隔后派若宁嗜色性的缺失不应影响伯基特淋巴瘤的诊断。
