Melnick J, Pickering M
Department of Chemistry, Princeton University, New Jersey 08544.
Biochem Int. 1988 Jan;16(1):69-75.
The displacement of methyl green, a dye bound ionically to double stranded DNA, has been suggested as a potential assay for drug-DNA interaction. The present investigation studies the equilibrium system of methyl green, its colorless carbinol form and DNA in the presence of salt (MgSO4), ethidium bromide, and quinine. The reversibility of the equilibrium, and the absence of sequestered sites or two modes of binding for methyl green are demonstrated.
甲基绿是一种通过离子键与双链DNA结合的染料，其位移已被提议作为药物与DNA相互作用的一种潜在检测方法。本研究考察了在盐（硫酸镁）、溴化乙锭和奎宁存在的情况下，甲基绿及其无色甲醇形式与DNA的平衡体系。证明了该平衡的可逆性，以及不存在甲基绿的隐蔽位点或两种结合模式。
