Nagy Anetta, Banyai Daniel, Semjen David, Beothe Tamas, Kovacs Gyula
Medical Faculty, University of Pecs, Pécs, Hungary.
Department of Urology, University of Pecs, Pécs, Hungary.
Virchows Arch. 2015;467:695-700. doi: 10.1007/s00428-015-1856-y. Epub 2015 Oct 16.
There are no adequate immunohistochemical markers for papillary renal cell tumours. The aim of this study was to establish a gene expression profile of papillary renal cell tumours using an expression microarray approach. Through hierarchical clustering and significant analysis of microarrays, we have selected the best 13 genes and analysed their expression by real-time polymerase chain reaction (RT-PCR). Of these genes, we selected SCEL as potential marker of interest. Immunohistochemical staining of tissue microarrays containing all major types of kidney cancers revealed positive staining for sciellin in 87 of 114 papillary renal cell tumours and in 13 of 19 precursor lesions. No other renal tumour types were positive for sciellin. Our study indicates that although not all tumours express sciellin, its expression may help to confirm the diagnosis papillary renal cell tumour.
目前尚无适用于乳头状肾细胞肿瘤的免疫组化标志物。本研究旨在采用表达微阵列方法建立乳头状肾细胞肿瘤的基因表达谱。通过层次聚类和微阵列的显著性分析，我们筛选出了13个最佳基因，并通过实时聚合酶链反应（RT-PCR）分析了它们的表达情况。在这些基因中，我们选择了SCEL作为潜在的感兴趣标志物。对包含所有主要类型肾癌的组织微阵列进行免疫组化染色，结果显示，在114例乳头状肾细胞肿瘤中的87例以及19例前驱病变中的13例中，sciellin呈阳性染色。其他类型的肾肿瘤均未检测到sciellin阳性。我们的研究表明，虽然并非所有肿瘤都表达sciellin，但其表达可能有助于确诊乳头状肾细胞肿瘤。
