Hasse Susanne, Hyman Anthony A, Sarov Mihail
Max Planck Institute of Molecular Cell Biology and Genetics Dresden, Germany.
Methods. 2016 Mar 1;96:69-74. doi: 10.1016/j.ymeth.2015.10.005. Epub 2015 Oct 17.
The localization of a protein is intrinsically linked to its role in the structural and functional organization of the cell. Advances in transgenic technology have streamlined the use of protein localization as a function discovery tool. Here we review the use of large genomic DNA constructs such as bacterial artificial chromosomes as a transgenic platform for systematic tag-based protein function exploration.
蛋白质的定位与其在细胞结构和功能组织中的作用有着内在联系。转基因技术的进步简化了蛋白质定位作为功能发现工具的应用。在此，我们综述了使用大型基因组DNA构建体（如细菌人工染色体）作为转基因平台进行基于标签的蛋白质功能系统探索的情况。
