大鼠肝微粒体中依赖鸟苷三磷酸（GTP）的钙离子释放。不需要囊泡融合。

GTP-dependent Ca2+ release from rat liver microsomes. Vesicle fusion is not required.

作者信息

Kleineke J, Schröder A, Söling H D

机构信息

Abteilung für Klin, Biochemie, Zentrum Innere Medizin der Universität, Göttingen, FRG.

出版信息

FEBS Lett. 1989 Mar 13;245(1-2):274-8. doi: 10.1016/0014-5793(89)80236-4.

DOI:10.1016/0014-5793(89)80236-4

PMID:2647523

Abstract

The GTP-dependent calcium release from rat liver microsomes is known to be promoted in the presence of colloids like polyethyleneglycol (PEG), polyvinylpyrrolidine, or albumin. Dawson et al. [(1987) Biochem. J. 244, 87-92] using the 'fusogen' PEG have concluded that both GTP-induced calcium efflux and the enhancement of InsP3-promoted calcium release in the presence of GTP could be attributed to a GTP-dependent vesicle fusion. Here, using the more physiological colloid albumin we report that GTP-induced calcium release from rat liver microsomes may not be linked to vesicle fusion.

摘要

已知在诸如聚乙二醇（PEG）、聚乙烯吡咯烷酮或白蛋白等胶体存在的情况下，大鼠肝微粒体中依赖GTP的钙释放会得到促进。道森等人[（1987年）《生物化学杂志》244卷，87 - 92页]使用“融合剂”PEG得出结论，GTP诱导的钙外流以及在GTP存在下肌醇三磷酸（InsP3）促进的钙释放增强都可归因于依赖GTP的囊泡融合。在此，我们使用更具生理性的胶体白蛋白报告称，GTP诱导的大鼠肝微粒体钙释放可能与囊泡融合无关。