Watanabe Tomohiro, Kojima Hisaya, Shinohara Arisa, Fukui Manabu
The Institute of Low Temperature Science, Hokkaido University, Kita-19, Nishi-8, Kita-ku, Sapporo 060-0819, Japan.
Int J Syst Evol Microbiol. 2016 Jan;66(1):113-117. doi: 10.1099/ijsem.0.000679. Epub 2015 Oct 15.
A novel sulfur-oxidizing bacterium, designated strain BiS0T, was isolated from a sediment sample collected from a freshwater lake in Japan. The cells were rod-shaped, 1.4-4.6 × 0.4-0.7 μm and Gram-stain-negative. The G+C content of the genomic DNA was around 44 mol%. The isolate possessed summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c), C16 : 0 and C10 : 0 3-OH as major cellular fatty acids. Strain BiS0T grew by carbon dioxide fixation and oxidation of inorganic sulfur compounds with oxygen as the electron acceptor. Growth was observed over a temperature range of 0-32 °C (optimum, 15-22 °C), an NaCl concentration range of 0-546.4 mM (optimum 0-66.7 mM) and a pH range of 5.2-8.1 (optimum 6.1-6.3). Phylogenetic analysis, based on 16S rRNA gene sequences, indicated that strain BiS0T belongs to the family Sulfuricellaceae in the class Betaproteobacteria. The closest cultured relatives were Sulfuricella denitrificans skB26T and Sulfuricella sp. T08, with 16S rRNA gene sequence similarities of 96.3 %. On the basis of the data obtained in this study, strain BiS0T represents a novel species of a novel genus, for which the name Sulfurirhabdus autotrophica gen. nov., sp. nov. is proposed. The type strain is BiS0T ( = NBRC 110941T = DSM 100309T).
从日本一个淡水湖采集的沉积物样本中分离出一种新型的硫氧化细菌，命名为菌株BiS0T。细胞呈杆状，大小为1.4 - 4.6×0.4 - 0.7μm，革兰氏染色阴性。基因组DNA的G + C含量约为44mol%。该分离株以 summed feature 3（C16 : 1ω7c和/或C16 : 1ω6c）、C16 : 0和C10 : 0 3 - OH作为主要细胞脂肪酸。菌株BiS0T通过二氧化碳固定以及以氧气作为电子受体氧化无机硫化合物来生长。在0 - 32°C（最适温度为15 - 22°C）的温度范围内、0 - 546.4mM（最适浓度为0 - 66.7mM）的NaCl浓度范围内以及5.2 - 8.1（最适pH为6.1 - 6.3）的pH范围内均观察到生长。基于16S rRNA基因序列的系统发育分析表明，菌株BiS0T属于β-变形菌纲中的硫细胞菌科。与其亲缘关系最近的已培养菌株是反硝化硫细胞菌skB26T和硫细胞菌属的T08，16S rRNA基因序列相似性为96.3%。根据本研究获得的数据，菌株BiS0T代表一个新属的新物种，为此提出新属名为自养硫杆状菌属（Sulfurirhabdus），新物种名为自养硫杆状菌（Sulfurirhabdus autotrophica）。模式菌株为BiS0T（= NBRC 110941T = DSM 100309T）。
