Wardach Jacob, Wagner Monica, Jeong Younhee, Holden Janean E
University of Michigan, Ann Arbor, USA.
Kyung Hee University, Seoul, South Korea.
West J Nurs Res. 2016 Mar;38(3):292-307. doi: 10.1177/0193945915610083. Epub 2015 Oct 15.
No evidence to date shows that lateral hypothalamic (LH) stimulation produces orexin-A-mediated antinociception in the spinal cord dorsal horn (SCDH) in a model of neuropathic pain. We conducted experiments to examine the effect of orexin-A-mediated LH stimulation in female rats with chronic constriction injury (CCI) on thermal hyperalgesia. Rats receiving carbachol into the LH demonstrated antinociception on both the left CCI and right nonligated paws (p < .05). Rats were given carbachol in the LH followed by intrathecal injection of the orexin-1 (OX1) receptor antagonist SB-334867, which blocked LH-induced antinociception compared with control groups (p < .05) in the left paw, but not in the right paw. These findings support the hypothesis that LH stimulation produces antinociception in rats with thermal hyperalgesia from neuropathic pain via an orexin-A connection between the LH and the SCDH. Identification of this pathway may lead to studies using orexins to manage clinical pain.
迄今为止，尚无证据表明在神经性疼痛模型中，下丘脑外侧区（LH）刺激能在脊髓背角（SCDH）产生食欲素A介导的抗伤害感受。我们进行了实验，以研究食欲素A介导的LH刺激对慢性压迫性损伤（CCI）雌性大鼠热痛觉过敏的影响。向LH注射卡巴胆碱的大鼠在左CCI爪和右未结扎爪上均表现出抗伤害感受（p < 0.05）。给LH注射卡巴胆碱后，再鞘内注射食欲素-1（OX1）受体拮抗剂SB-334867，与对照组相比，该拮抗剂阻断了LH诱导的左爪抗伤害感受（p < 0.05），但右爪未受影响。这些发现支持了这样的假说：LH刺激通过LH与SCDH之间的食欲素A连接，在患有神经性疼痛所致热痛觉过敏的大鼠中产生抗伤害感受。确定这一途径可能会促使开展使用食欲素治疗临床疼痛的研究。
