如果在再次培养过程中避免解冻，流感嗜血杆菌分离株在脱脂乳胰蛋白胨葡萄糖甘油肉汤（STGGB）中于-70°C可存活长达20年。
Haemophilus influenzae isolates survive for up to 20 years at -70 °C in skim milk tryptone glucose glycerol broth (STGGB) if thawing is avoided during re-culture.
作者信息
Hare K M, Smith-Vaughan H C, Beissbarth J, Leach A J
机构信息
Menzies School of Health Research, PO Box 41096, Casuarina, NT 0811, Australia.
Menzies School of Health Research, PO Box 41096, Casuarina, NT 0811, Australia.
出版信息
J Microbiol Methods. 2015 Dec;119:132-3. doi: 10.1016/j.mimet.2015.10.013. Epub 2015 Oct 22.
Haemophilus influenzae remains a major cause of disease worldwide requiring continued study. Recently, isolates of Streptococcus pneumoniae and Moraxella catarrhalis, but not H. influenzae, were reported to survive long-term ultra-freeze storage in STGGB. We show that nontypeable H. influenzae isolates survive for up to 20 years when thawing is avoided.
流感嗜血杆菌仍是全球范围内需要持续研究的主要致病原因。最近，有报道称肺炎链球菌和卡他莫拉菌的分离株能在STGGB中经长期超低温冷冻保存存活，但流感嗜血杆菌不能。我们发现，避免解冻的情况下，不可分型流感嗜血杆菌分离株可存活长达20年。
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