Flournoy D J, Jones J B
Zhonghua Min Guo Wei Sheng Wu Ji Mian Yi Xue Za Zhi. 1985 Aug;18(3):184-9.
Studies were done on clinical isolates of Haemophilus influenzae to investigate viability and determine the effects of disc-agar diffusion (DAD) medium modification on antimicrobial susceptibility results. Most isolates were viable for two days in distilled water, up to a week on chocolate agar and months when frozen in skim milk at -70 degrees C. Differences in viability were not related to biotype, serotype, beta-lactamase production or site of isolation of isolates. Several medium modifications resulted in better growth of isolates for antimicrobial susceptibility testing by DAD, but the zone sizes of inhibition differed from those of the recommended medium.
对流感嗜血杆菌的临床分离株进行了研究，以调查其活力，并确定纸片琼脂扩散法（DAD）培养基改良对抗菌药物敏感性结果的影响。大多数分离株在蒸馏水中可存活两天，在巧克力琼脂上可存活长达一周，在-70℃的脱脂牛奶中冷冻时可存活数月。活力差异与分离株的生物型、血清型、β-内酰胺酶产生或分离部位无关。几种培养基改良方法使分离株在DAD抗菌药物敏感性试验中生长得更好，但抑菌圈大小与推荐培养基的不同。
