Caron J M
Department of Medicine, University of California, San Francisco 94143.
J Cell Physiol. 1989 Mar;138(3):603-10. doi: 10.1002/jcp.1041380322.
Incubation of primary cultures of rat hepatocytes with insulin caused an alteration in several parameters of microtubule physiology. There was a transient decrease in total cellular tubulin levels and, at the same time, an apparent stabilization of microtubule polymers which was also transient. The level of microtubule polymer, however, was not altered, which indicated that an insulin-inducible decrease in the level of nonpolymerized tubulin was responsible for the decrease in total cellular tubulin. These changes were followed by an increase in tubulin mRNA levels and, shortly thereafter, by an increase in tubulin protein synthesis. This study demonstrates that microtubule physiology in hepatocytes is modulated by insulin and, further, suggests a model by which insulin-induced changes in microtubule physiology may play a role in regulating other insulin responses.
用胰岛素孵育大鼠肝细胞原代培养物会导致微管生理学的几个参数发生改变。总细胞微管蛋白水平出现短暂下降，与此同时，微管聚合物出现明显的稳定状态，这种稳定也是短暂的。然而，微管聚合物的水平没有改变，这表明非聚合微管蛋白水平的胰岛素诱导性下降是总细胞微管蛋白下降的原因。这些变化之后是微管蛋白mRNA水平的增加，此后不久，微管蛋白蛋白质合成也增加。这项研究表明，肝细胞中的微管生理学受到胰岛素的调节，并且进一步提出了一个模型，通过该模型胰岛素诱导的微管生理学变化可能在调节其他胰岛素反应中发挥作用。
