Tawhid H, Labastide W, Barker C, Rees J
Department of Haematological Medicine, University of Cambridge Clinical School, U.K.
Leuk Res. 1989;13(2):127-30. doi: 10.1016/0145-2126(89)90136-7.
Human acute erythroleukaemia arises from the inability of the haemopoietic stem cell to differentiate. K 562 cell line provides a homogeneous population of primitive erythroleukaemic cells that are at the same point of differentiation. The effect of human recombinant granulocyte-macrophage colony-stimulating factor and human recombinant erythropoietin on the differentiation of K 562 clonogenic cells was studied. Cells were cultured in methylcellulose culture for 5 days at 37 degrees C in humidified atmosphere containing 5% CO2 in air and scored for erythroid differentiation by benzidine staining. A combination of both growth factors induced erythroid differentiation in more than 80% of K 562 clonogenic cells. This combination may be useful in the treatment of patients with erythroleukaemia.
人类急性红白血病源于造血干细胞无法分化。K562细胞系提供了处于相同分化阶段的原始红白血病细胞的同质群体。研究了重组人粒细胞巨噬细胞集落刺激因子和重组人促红细胞生成素对K562克隆形成细胞分化的影响。细胞在含有5%二氧化碳的湿润空气中，于37℃在甲基纤维素培养基中培养5天，并用联苯胺染色对红系分化进行评分。两种生长因子的组合可诱导超过80%的K562克隆形成细胞发生红系分化。这种组合可能对红白血病患者的治疗有用。
